Department of Pharmacology, Faculty of Pharmacy, Erzincan Binali Yildirim University, Erzincan, 24100, Turkey.
Department of Pharmacology, Faculty of Medicine, Ataturk University, Erzurum, 25240, Turkey.
J Mol Neurosci. 2021 May;71(5):1095-1111. doi: 10.1007/s12031-020-01732-3. Epub 2020 Nov 16.
Although several pieces of evidence have indicated the ability of the serotonin-7 receptor (5-HTR) to modulate N-methyl--aspartate receptor (NMDAR) activation, the possible impact on ketamine anesthesia has not been examined directly. The purpose of the present study is thus to investigate the possible role of the 5-HTR in ketamine anesthesia using a 5-HTR agonist and/or antagonist. The influence of a 5-HTR agonist/antagonist on ketamine anesthesia for behavioral impact was assessed by testing potential anesthetic parameters. Its functional impact was assessed by mRNA expression with real-time PCR and immunostaining in the hippocampus and prefrontal cortex of mice. Two different doses of ketamine-high and low-were administered to induce anesthesia. In the high-dose ketamine-applied group in particular, the administration of both the 5-HTR agonist and antagonist intensified the anesthetic effect of ketamine. The reflection of the change in anesthesia parameters to 5-HTR expression was observed as an increase in the hippocampus and a decrease in the prefrontal cortex in the anesthetized groups by stimulation of 5-HTR. It is noteworthy that the results of NMDAR expressions are parallel to the results of the 5-HTR expressions of both the hippocampus and the prefrontal cortex. The 5-HTR may play a role in ketamine anesthesia. It may act through NMDAR in ketamine anesthesia, depending on the parallelism between both receptors.
尽管有几项证据表明 5-羟色胺-7 受体（5-HTR）能够调节 N-甲基-D-天冬氨酸受体（NMDAR）的激活，但尚未直接研究其对氯胺酮麻醉的可能影响。因此，本研究旨在使用 5-HTR 激动剂和/或拮抗剂来研究 5-HTR 在氯胺酮麻醉中的可能作用。通过测试潜在的麻醉参数来评估 5-HTR 激动剂/拮抗剂对氯胺酮麻醉行为影响的影响。通过实时 PCR 和免疫染色在小鼠海马体和前额叶皮层中评估其功能影响。给予两种不同剂量的氯胺酮-高和低-以诱导麻醉。特别是在高剂量氯胺酮应用组中，5-HTR 激动剂和拮抗剂的联合给药增强了氯胺酮的麻醉效果。通过刺激 5-HTR，观察到麻醉参数变化对 5-HTR 表达的反射，表现为麻醉组中海马体的增加和前额叶皮层的减少。值得注意的是，NMDAR 表达的结果与海马体和前额叶皮层中 5-HTR 表达的结果平行。5-HTR 可能在氯胺酮麻醉中发挥作用。它可能通过 NMDAR 在氯胺酮麻醉中起作用，这取决于两种受体之间的平行性。
