Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
Mahidol University and Osaka Collaborative Research Center on Bioscience and Biotechnology, Bangkok 10400, Thailand.
Int J Syst Evol Microbiol. 2020 May;70(5):3069-3075. doi: 10.1099/ijsem.0.004136.
A novel marine actinomycete, designated strain KJ-029, was isolated from a marine sediment sample (water depth of 226 m) in Kagoshima, Japan. 16S rRNA gene sequence analysis revealed that the new isolate was most closely related to LHW 63014 (99.3 % similarity). Phylogenetic analyses of the genus based on 16S rRNA gene sequences showed that strain KJ-029 was clustered with LHW 63014 and 202201. However, digital DNA-DNA hybridization analyses presented low levels of relatedness in the range of 24.8-32.9 % between strain KJ-029 and the above closely related strains. The novel strain contained -diaminopimelic acid and 3-OH-diaminopimelic acid, d-glutamic acid, glycine and d-alanine in the cell-wall peptidoglycan. The acyl type of the peptidoglycan was glycolyl and mycolic acids were absent. The major menaquinone was MK-9(H). The whole-cell sugars consisted of glucose, mannose, xylose and ribose. Phosphatidylethanolamine was detected as the major phospholipid and corresponded to phospholipid type II. The predominant cellular fatty acid was iso-C. The DNA G+C content of the genomic DNA was 71.5 mol%. Based on the present polyphasic study, strain KJ-029 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KJ-029 (=NBRC 113519=TBRC 9233).
一株新型海洋放线菌，命名为菌株 KJ-029，从日本鹿儿岛县水深 226 米的海洋沉积物样本中分离得到。16S rRNA 基因序列分析表明，新分离株与 LHW 63014（相似度 99.3%）最为密切相关。基于 16S rRNA 基因序列的属内系统发育分析表明，菌株 KJ-029与 LHW 63014 和 202201 聚类在一起。然而，菌株 KJ-029 与上述密切相关菌株之间的数字 DNA-DNA 杂交分析显示，其相关性水平较低，在 24.8-32.9%之间。新型菌株的细胞壁肽聚糖中含有 -二氨基庚二酸和 3-OH-二氨基庚二酸、d-谷氨酸、甘氨酸和 d-丙氨酸。肽聚糖的酰基类型为甘醇酰基，不存在类脂酰基。主要的menaquinone 是 MK-9(H)。全细胞糖由葡萄糖、甘露糖、木糖和核糖组成。磷脂酰乙醇胺被检测为主要的磷脂，对应于磷脂类型 II。主要的细胞脂肪酸是 iso-C。基因组 DNA 的 G+C 含量为 71.5 mol%。基于目前的多相研究，菌株 KJ-029 代表了 属的一个新型种，建议将其命名为 sp. nov.。模式菌株为 KJ-029（=NBRC 113519=TBRC 9233）。
