Lab of Veterinary Biological Products, College of Animal Science and Technology, Henan University of Science and Techology, China.
Lab of Medical Microbiological Engineering, College of Medical Technology and Engineering, Henan University of Science and Techology, Luoyang, China.
Microb Pathog. 2019 Oct;135:103620. doi: 10.1016/j.micpath.2019.103620. Epub 2019 Jul 13.
NDM-1-producing Enterobacteriaceae are multidrug-resistant bacteria, also called superbacteria, that have become important global human health threats in recent years. However, data about NDM-1-producing bacteria in animals are rare. In this study, an NDM-1-producing Escherichia coli isolate (designated E120413) was obtained from pigs in Henan province, China in 2012. The susceptibility of E. coli E120413 to antimicrobial agents was determined using Kirby-Bauer disk diffusion and micro-dilution methods. Susceptibility tests indicated that E. coli E120413 was resistant to almost all common antibiotics with high MIC values obtained for most antibiotics tested. E. coli E120413 was detected in the heart, liver, spleen, lung, kidney, brain, stomach, duodenum, mesenteric lymph nodes, and fecal samples of piglets in both cohabitation and experimental groups and the bacteria persisted for more than 2 weeks. However, no obvious clinical symptoms or serious pathological lesions were observed. This is the first investigation of NDM-1-producing E. coli isolate from pigs in China. Although no significant pathological lesions were observed, NDM-1-producing E. coli was found to be highly transmissible and to cause persistent infection in pigs.
产 NDM-1 肠杆菌科细菌是一种多药耐药菌，也被称为超级细菌，近年来已成为全球人类健康的重要威胁。然而，关于动物中产 NDM-1 细菌的数据却很少。本研究从 2012 年中国河南省的猪中分离到一株产 NDM-1 的大肠杆菌（命名为 E120413）。采用 Kirby-Bauer 纸片扩散法和微量稀释法测定 E. coli E120413 对抗菌药物的敏感性。药敏试验表明，E. coli E120413 对几乎所有常见抗生素均有耐药性，大多数受试抗生素的 MIC 值均较高。在同居和实验组的仔猪的心、肝、脾、肺、肾、脑、胃、十二指肠、肠系膜淋巴结和粪便样本中均检测到 E. coli E120413，且细菌持续存在超过 2 周。然而，未观察到明显的临床症状或严重的病理损伤。这是首次在中国从猪中分离到产 NDM-1 的大肠杆菌。尽管未观察到明显的病理损伤，但产 NDM-1 的大肠杆菌具有高度的传染性，并可导致猪持续性感染。
