Renzhammer René, Loncaric Igor, Roch Franz-Ferdinand, Pinior Beate, Käsbohrer Annemarie, Spergser Joachim, Ladinig Andrea, Unterweger Christine
University Clinic for Swine, University of Veterinary Medicine, 1210 Vienna, Austria.
Institute of Microbiology, University of Veterinary Medicine, 1210 Vienna, Austria.
Antibiotics (Basel). 2020 Apr 24;9(4):208. doi: 10.3390/antibiotics9040208.
Increasing numbers of multi-resistant from clinical specimens emphasize the importance of monitoring of their resistance profiles for proper treatment. Furthermore, knowledge on the presence of virulence associated genes in isolates from European swine stocks is scarce. Consequently, a total of 694 isolated between 2016 and 2018 from diarrheic piglets of Austrian swine herds were investigated. The isolates were tested for their susceptibility to twelve antibiotics using agar disk diffusion test and for the presence of 22 virulence associated genes via PCR. Overall, 71.9, 67.7, and 49.5% of all isolates were resistant to ampicillin, tetracycline, and trimethoprim-sulfamethoxazole, while resistance levels to gentamicin and fosfomycin were 7.7 and 2.0%, respectively. Resistance frequency to ciprofloxacin was higher than in previous studies. Isolates were more likely to be resistant to ampicillin if they were also resistant to ciprofloxacin. No isolate was resistant to meropenem or amikacin. Virulence genes were detected more frequently in isolates expressing hemolytic activity on blood agar plates. The detection rate of was increased in negative isolates. We assume, that hemolytic activity and absence of could be considered as potential indicators for the virulence of in piglets.
临床样本中多重耐药菌数量的增加凸显了监测其耐药谱以进行恰当治疗的重要性。此外，关于欧洲猪群分离株中与毒力相关基因存在情况的知识匮乏。因此，对2016年至2018年间从奥地利猪群腹泻仔猪中分离出的总共694株菌进行了研究。使用琼脂纸片扩散试验检测这些分离株对12种抗生素的敏感性，并通过聚合酶链反应检测22种与毒力相关基因的存在情况。总体而言，所有分离株中分别有71.9%、67.7%和49.5%对氨苄西林、四环素和甲氧苄啶 - 磺胺甲恶唑耐药，而对庆大霉素和磷霉素的耐药水平分别为7.7%和2.0%。对环丙沙星的耐药频率高于先前研究。如果分离株对环丙沙星耐药，那么它更有可能对氨苄西林耐药。没有分离株对美罗培南或阿米卡星耐药。在血琼脂平板上表现出溶血活性的分离株中，毒力基因的检测更为频繁。在阴性分离株中，的检出率有所增加。我们认为，溶血活性和的缺失可被视为仔猪中毒力的潜在指标。
