Faculty of Biotechnology, University of Agronomic Science and Veterinary Medicine, 59 Marasti blvd, 1 District, 011464 Bucharest, Romania.
Department of Genetics, ICUB-Research Institute of the University of Bucharest, 36-46 Bd. M. Kogalniceanu, 5th District, 050107 Bucharest, Romania.
Genes (Basel). 2019 Jul 15;10(7):535. doi: 10.3390/genes10070535.
Non-nutritive sweeteners represent an ingredient class that directly affects human health, via the development of inflammatory processes that promote chronic diseases related to microbiota dysbiosis. Several in vitro tests were conducted in the static GIS1 simulator. The aim of the study was to highlight the effect of sweeteners on the microbiota pattern of healthy individuals, associated with any alteration in the metabolomic response, through the production of organic acids and ammonium. The immediate effect of the in vitro treatment and the influence of the specific sweetener type on the occurrence of dysbiosis were evaluated by determining the biomarkers of the microbiota response. The presence of the steviol reduced the ammonium level (minimum of 410 mg/L), while the addition of cyclamate and saccharin caused a decrease in the number of microorganisms, in addition to lowering the total quantity of synthesized short-chain fatty acids (SCFAs). The bifidobacteria appeared to decrease below 10 genomes/mL in all the analyzed samples at the end of the in vitro simulation period. Barring the in vitro treatment of steviol, all the sweeteners tested exerted a negative influence on the fermentative profile, resulting in a decline in the fermentative processes, a rise in the colonic pH, and uniformity of the SCFA ratio.
非营养性甜味剂是一类直接影响人类健康的成分，通过引发炎症过程促进与微生物失调相关的慢性疾病。在静态 GIS1 模拟器中进行了几项体外测试。本研究的目的是通过产生有机酸和氨来强调甜味剂对健康个体微生物群模式的影响，以及任何代谢组学反应的改变。通过确定微生物群反应的生物标志物来评估体外处理的即时影响和特定甜味剂类型对失调发生的影响。甜菊醇的存在降低了氨的水平（最低 410mg/L），而环氨酸和糖精的添加除了降低合成的短链脂肪酸 (SCFA) 的总量外，还减少了微生物的数量。在体外模拟期末，双歧杆菌在所有分析样本中的数量似乎都降至 10 个基因组/mL 以下。除了甜菊醇的体外处理外，所有测试的甜味剂都对发酵谱产生了负面影响，导致发酵过程下降、结肠 pH 值上升和 SCFA 比例均匀。
