Liu Wenjuan, Huan Zhiguang, Xing Min, Tian Tian, Xia Wei, Wu Chengtie, Zhou Zhihua, Chang Jiang
School of Materials Science and Engineering, Hunan University of Science and Technology, Xiangtan 411201, China.
Division of Applied Materials Science, Department of Engineering Sciences, The Ångström Laboratory, Uppsala University, SE-751 21 Uppsala, Sweden.
Materials (Basel). 2019 Jul 15;12(14):2276. doi: 10.3390/ma12142276.
Incorporating Sr element in biomaterials for bone implants is an effective way to improve their biological performance, as Sr element has been proved to enhance bone regeneration and depress bone resorption activity. In the present study, we developed a Sr-incorporated dicalcium silicate (C2S) bone cement as a potential candidate for bioactive self-setting bone cement in orthopaedics and stomatology. The Sr-C2S powders containing 0.3-6.8% Sr in molar ratio were prepared by means of chemical co-precipitation, and the results of XRD analysis indicated the incorporation of Sr element into the lattice of C2S. Sr-C2S bone cements, as prepared by mixing the powders with water, have a final setting time of 570 to 594 min, and compressive strength higher than that of C2S bone cement within certain incorporation range. The Sr-C2S bone cements possessed good in vitro bioactivity by inducing apatite formation in simulated body fluid (SBF) within 7 days. Moreover, the proliferation activity of human bone marrow mesenchymal stem cells (hBMSCs) with Sr-C2S bone cements was significantly higher than that with C2S bone cement, and the alkaline phosphatase (ALP) activity of hBMSCs was also enhanced with addition of Sr element in Sr-C2S groups. The Sr-C2S might therefore be a bioactive self-setting material with enhanced biological performance and holds the prospect for application in the bone regeneration area.
在骨植入生物材料中掺入锶元素是提高其生物学性能的有效方法，因为已证明锶元素可促进骨再生并抑制骨吸收活性。在本研究中，我们开发了一种掺入锶的硅酸二钙（C2S）骨水泥，作为骨科和口腔科生物活性自固化骨水泥的潜在候选材料。通过化学共沉淀法制备了摩尔比含0.3 - 6.8%锶的Sr-C2S粉末，XRD分析结果表明锶元素已掺入C2S晶格中。将这些粉末与水混合制备的Sr-C2S骨水泥，最终凝固时间为570至594分钟，在一定掺入范围内抗压强度高于C2S骨水泥。Sr-C2S骨水泥在模拟体液（SBF）中7天内可诱导磷灰石形成，具有良好的体外生物活性。此外，Sr-C2S骨水泥培养的人骨髓间充质干细胞（hBMSCs）的增殖活性明显高于C2S骨水泥培养的，并且在Sr-C2S组中添加锶元素后hBMSCs的碱性磷酸酶（ALP）活性也增强。因此，Sr-C2S可能是一种具有增强生物学性能的生物活性自固化材料，在骨再生领域具有应用前景。
