Functional DNA variations associated with Saudi female with low VO: a pilot microarray study.

The study aims to explore the genetic predispositions and molecular pathways of low cardiorespiratory fitness (VO) in young Saudi females ( = 70). Young females were grouped based on the level of VO according to the specification of the physical fitness specialist certification as low VO (< 28.9; = 19) and high VO (> 33; = 14) and genotyped for 243345 putative functional exonic variants. The -rs10037485T, -rs75380747G and -rs13695C SNPs on chromosome 5, 12 and 17, respectively were found to be the most significant among young Saudi females with low VO ( < 8.01 × 10). Linkage disequilibrium (LD) analysis among the significant SNPs ( < 0.001) have revealed risk and protective haplotypes with high degree (-value < 1.0 × 10) of LD. The most significant risk haplotypes with the low VO in young Saudi females are: Chromosome 1: -rs10800201G; -rs4657537A; rs4657583T (-value = 2.00 × 10); Chromosome 3: rs978979G; -rs7637449A; -rs111934125T; -rs9835332G (-value = 5.00 × 10) and Chromosome 17: -rs13696C; -rs1901187C (-value = 1.00 × 10). Functional Enrichment Analysis revealed that the genes with SNPs < 0.001 have significantly involved in the heart rate ( = 0.00442), body weight ( = 0.00629), breath tests ( = 0.0147), proteolysis ( = 0.00623) and cardiac muscle fiber development ( = 0.0263). In conclusion we could say that the identified genetic predispositions and gene-annotation enrichment in low VO in young Saudi females revealed that they are at high risk for developing cardiovascular complications.