Czerkinsky C, Andersson G, Ekre H P, Nilsson L A, Klareskog L, Ouchterlony O
Department of Oral Microbiology, University of Göteborg, Sweden.
J Immunol Methods. 1988 May 25;110(1):29-36. doi: 10.1016/0022-1759(88)90079-8.
A reverse modification of ELISPOT assay using nitrocellulose membranes and epitope-specific monoclonal antibodies is described for the detection of single lymphokine-secreting cells. As a model, the production of gamma-interferon by mitogen stimulated human peripheral blood lymphocytes has been examined. The assay can also be modified to permit microscopic examination of spot-forming cells.
描述了一种使用硝酸纤维素膜和表位特异性单克隆抗体对ELISPOT分析进行的反向改良方法，用于检测单个分泌淋巴因子的细胞。作为一个模型，已检测了丝裂原刺激的人外周血淋巴细胞产生γ-干扰素的情况。该分析也可进行改良，以便对斑点形成细胞进行显微镜检查。
