Ph.D Program in Dental Biomedical Sciences, University of Maryland School of Dentistry, Baltimore, MD 21201, USA.
Department of Preventive Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam 34212, Saudi Arabia.
Int J Mol Sci. 2019 Jul 16;20(14):3491. doi: 10.3390/ijms20143491.
Cariogenic oral biofilms are strongly linked to dental caries around dental sealants. Quaternary ammonium monomers copolymerized with dental resin systems have been increasingly explored for modulation of biofilm growth. Here, we investigated the effect of dimethylaminohexadecyl methacrylate (DMAHDM) on the cariogenic pathogenicity of () biofilms. DMAHDM at 5 mass% was incorporated into a parental formulation containing 20 mass% nanoparticles of amorphous calcium phosphate (NACP). biofilms were grown on the formulations, and biofilm inhibition and virulence properties were assessed. The tolerances to acid stress and hydrogen peroxide stress were also evaluated. Our findings suggest that incorporating 5% DMAHDM into 20% NACP-containing sealants (1) imparts a detrimental biological effect on by reducing colony-forming unit counts, metabolic activity and exopolysaccharide synthesis; and (2) reduces overall acid production and tolerance to oxygen stress, two major virulence factors of this microorganism. These results provide a perspective on the value of integrating bioactive restorative materials with traditional caries management approaches in clinical practice. Contact-killing strategies via dental materials aiming to prevent or at least reduce high numbers of cariogenic bacteria may be a promising approach to decrease caries in patients at high risk.
致龋口腔生物膜与牙釉质封闭剂周围的龋齿密切相关。季铵盐单体与牙科树脂体系共聚，已被越来越多地用于调节生物膜的生长。在这里，我们研究了二甲基氨基己基甲基丙烯酸酯（DMAHDM）对（）生物膜致龋致病性的影响。5%质量的 DMAHDM 被掺入含有 20%质量纳米无定形磷酸钙（NACP）的母体配方中。在制剂上培养生物膜，并评估生物膜抑制和毒力特性。还评估了对酸应激和过氧化氢应激的耐受性。我们的研究结果表明，将 5% DMAHDM 掺入含有 20% NACP 的密封剂中（1）通过减少菌落形成单位计数、代谢活性和胞外多糖合成，对（）产生有害的生物学影响；（2）降低了这种微生物的主要毒力因子——总产酸量和耐氧应激能力。这些结果为将生物活性修复材料与传统龋病管理方法相结合在临床实践中的价值提供了一个视角。通过牙科材料进行接触杀伤的策略，旨在预防或至少减少致龋细菌的数量，可能是减少高危患者龋齿的一种有前途的方法。
