Ibrahim Maria Salem, Alabbas Mana'a S, Alsomaly Khalid U, AlMansour Abdullah A, Aljouie Alhareth Abdulaziz, Alzahrani Majed M, Asseri Ahmed A, AlHumaid Jehan
Department of Preventive Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam 34212, Saudi Arabia.
College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam 34212, Saudi Arabia.
Polymers (Basel). 2021 Dec 24;14(1):61. doi: 10.3390/polym14010061.
To assess the remineralizing abilities and compare the flexural strength and elastic modulus of different bioactive pit and fissure sealants.
Human enamel samples were randomly and blindly sealed with one of the following bioactive materials: BioCoat (Bc), ACTIVA KIDS (Av) and BeautiSealant (Bu). Seal-it (Si) was used as a non-bioactive sealant beside a control blank (B) group with no sealant. The sealed samples were subjected to a pH-cycling model (7 days of demineralization-remineralization cycles). The enamel surface hardness change (SHC), scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDX) and polarized light microscopy were used to assess the remineralizing abilities of the studied sealants. Flexural strength and elastic modulus were also assessed following the ISO 4049 protocols. One-way analysis of variance (ANOVA) was used to analyze the results.
Bc sealant showed the highest FS and EM ( < 0.05). The contact with Bc and Bu sealants showed significantly lower %SHL ( < 0.05) in comparison to the other. These findings were supported by the results of SEM-EDX and polarized imaging by showing higher percentages of calcium and phosphate ions with the former sealants and thinner demineralized enamel bands.
In this study, Bc showed the highest flexural strength. Bc and Bu sealants outperformed the other studied sealants in terms of their remineralization abilities.
评估不同生物活性窝沟封闭剂的再矿化能力，并比较其弯曲强度和弹性模量。
将人类牙釉质样本随机且盲法地用以下生物活性材料之一进行封闭：BioCoat（Bc）、ACTIVA KIDS（Av）和BeautiSealant（Bu）。除了未进行封闭的空白对照组（B）外，将Seal-it（Si）用作非生物活性封闭剂。对封闭后的样本进行pH循环模型（7天的脱矿化-再矿化循环）处理。使用牙釉质表面硬度变化（SHC）、扫描电子显微镜-能量色散X射线光谱仪（SEM-EDX）和偏光显微镜来评估所研究封闭剂的再矿化能力。还按照ISO 4049标准评估弯曲强度和弹性模量。采用单因素方差分析（ANOVA）对结果进行分析。
Bc封闭剂显示出最高的FS和EM（<0.05）。与其他封闭剂相比，Bc和Bu封闭剂接触后的%SHL显著更低（<0.05）。SEM-EDX结果和偏光成像结果支持了这些发现，即前一种封闭剂显示出更高百分比的钙和磷酸根离子，且脱矿化牙釉质带更薄。
在本研究中，Bc显示出最高的弯曲强度。Bc和Bu封闭剂在再矿化能力方面优于其他所研究的封闭剂。
