Hu Yifeng, Yan Jingtie
Department of Pediatric Surgery, Zaozhuang Municipal Hospital, Zaozhuang, Shandong 277102, P.R. China.
Exp Ther Med. 2019 Aug;18(2):1021-1028. doi: 10.3892/etm.2019.7643. Epub 2019 Jun 4.
Nephroblastoma is the most common renal tumor in children. Abnormal expression of microRNAs (miRs) has been reported to be involved in the progression of various types of cancers. However, the role and underlying mechanism of miR-130b-3p in nephroblastoma remains unknown. Therefore, the present study aimed to explore the role and possible mechanism of miR-130b-3p in nephroblastoma in children. The present study identified that miR-130b-3p was highly expressed in nephroblastoma tissues obtained from children with nephroblastoma. To better understand the functions and the molecular mechanisms of miR-130b-3p in nephroblastoma, TargetScan was used to identify the potential targets of miR-130b-3p. Phosphatase and tensin homolog (PTEN), was identified as a target gene of miR-130b-3p, and it was observed to be downregulated in nephroblastoma. Further analysis indicated that miR-130b-3p inhibitor could significantly reduce cell proliferation, induce apoptosis and suppress the Akt/nuclear factor-κB/survivin signaling pathway in nephroblastoma cells. Notably, all these effects of miR-130b-3p on nephroblastoma cells were reversed by PTEN-small interfering RNA. In summary, the present study suggested that the miR-130b-3p/PTEN axis could serve a critical role in the progression and development of nephroblastoma. It also suggests that miR-130b-3p might be a valuable clinical biomarker and therapeutic target for nephroblastoma in children.
肾母细胞瘤是儿童最常见的肾脏肿瘤。据报道，微小RNA（miR）的异常表达与各类癌症的进展有关。然而，miR-130b-3p在肾母细胞瘤中的作用及潜在机制仍不清楚。因此，本研究旨在探讨miR-130b-3p在儿童肾母细胞瘤中的作用及可能机制。本研究发现，miR-130b-3p在从患有肾母细胞瘤的儿童获取的肾母细胞瘤组织中高表达。为了更好地了解miR-130b-3p在肾母细胞瘤中的功能和分子机制，使用TargetScan来鉴定miR-130b-3p的潜在靶标。磷酸酶和张力蛋白同源物（PTEN）被鉴定为miR-130b-3p的靶基因，并且观察到其在肾母细胞瘤中表达下调。进一步分析表明，miR-130b-3p抑制剂可显著降低肾母细胞瘤细胞的增殖、诱导凋亡并抑制Akt/核因子-κB/生存素信号通路。值得注意的是，miR-130b-3p对肾母细胞瘤细胞的所有这些作用均被PTEN小干扰RNA逆转。总之，本研究表明miR-130b-3p/PTEN轴可能在肾母细胞瘤的进展和发展中起关键作用。这也表明miR-130b-3p可能是儿童肾母细胞瘤有价值的临床生物标志物和治疗靶点。
