Palozi Rhanany Alan Calloi, Guarnier Lucas Pires, Romão Paulo Vitor Moreira, Nocchi Samara Requena, Dos Santos Carlos Calixto, Lourenço Emerson Luiz Botelho, Silva Denise Brentan, Gasparotto Francielly Mourão, Gasparotto Junior Arquimedes
Laboratório de Eletrofisiologia e Farmacologia Cardiovascular (LEFaC), Faculdade de Ciências da Saúde, Universidade Federal da Grande Dourados, Dourados, MS, Brazil.
Laboratório de Produtos Naturais e Espectrometria de Massas (LaPNEM), Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição (FACFAN), Universidade Federal do Mato Grosso do Sul, Campo Grande, MS, Brazil.
Toxicol Rep. 2019 Jun 28;6:616-624. doi: 10.1016/j.toxrep.2019.06.017. eCollection 2019.
Fruit peels of (Mart.) Kausel are widely used in Brazilian traditional medicine, but no studies have proved the safety of its pharmacological effects on the respiratory, cardiovascular, and central nervous systems. The present study assessed the safety pharmacology of in New Zealand rabbits. First, an ethanol extract (EEPC) was selected for the pharmacological experiments and chemical characterization. Then, different groups of rabbits were orally treated with EEPC (200 and 2000 mg/kg) or vehicle. Acute behavioral and physiological alterations in the modified Irwin test, respiratory rate, arterial blood gas, and various cardiovascular parameters (i.e., heart rate, blood pressure, and electrocardiography) were evaluated. The main secondary metabolites that were identified in EEPC were ellagic acid, gallic acid, -deoxyhexosyl quercetin, and the anthocyanin -hexosyl cyanidin. No significant behavioral or physiological changes were observed in any of the groups. None of the doses of EEPC affected respiratory rate or arterial blood gas, with no changes on blood pressure or electrocardiographic parameters. The present study showed that EEPC did not cause any significant changes in respiratory, cardiovascular, or central nervous system function. These data provide scientific evidence of the effects of this species and important safety data for its clinical use.
考塞尔（Mart.）的果皮在巴西传统医学中被广泛使用，但尚无研究证明其对呼吸、心血管和中枢神经系统的药理作用的安全性。本研究评估了考塞尔在新西兰兔中的安全药理学。首先，选择乙醇提取物（EEPC）进行药理实验和化学表征。然后，用EEPC（200和2000mg/kg）或赋形剂对不同组的兔子进行口服治疗。评估改良欧文试验中的急性行为和生理改变、呼吸频率、动脉血气以及各种心血管参数（即心率、血压和心电图）。在EEPC中鉴定出的主要次生代谢产物是鞣花酸、没食子酸、-脱氧己糖基槲皮素和花青素-己糖基花青素。在任何一组中均未观察到明显的行为或生理变化。EEPC的任何剂量均未影响呼吸频率或动脉血气，血压或心电图参数也无变化。本研究表明，EEPC不会引起呼吸、心血管或中枢神经系统功能的任何显著变化。这些数据为该物种的作用提供了科学证据，并为其临床应用提供了重要的安全数据。
