University of Veterinary and Animal Sciences, Lahore, 54600, Pakistan.
Division of Biomedical and Life Sciences, Lancaster University, Lancaster, LA1 4YG, UK.
Mol Biol Rep. 2019 Oct;46(5):5587-5593. doi: 10.1007/s11033-019-04973-7. Epub 2019 Jul 17.
Peste des petits ruminants virus (PPRV) infects a wide range of domestic and wild ruminants, and occasionally unusual hosts such as camel, cattle and pig. Given their broad host-spectrum and disease endemicity in several developing countries, it is imperative to elucidate the viral evolutionary insights for their dynamic pathobiology and differential host-selection. For this purpose, a dataset of all available (n = 37) PPRV sequences originating from wild and unusual hosts was composed and in silico analysed. Compared to domestic small ruminant strains of same geographical region, phylogenomic and residue analysis of PPRV sequences originating from wild and unusual hosts revealed a close relationship between strains. A lack of obvious difference among the studied sequences and deduced residues suggests that these are the host factors that may play a role in their susceptibility to PPRV infection, immune response, pathogenesis, excretion patterns and potential clinical signs or resistance to clinical disease. Summarizing together, the comparative analysis enhances our understanding towards molecular epidemiology of the PPRV in wild and unusual hosts for appropriate intervention strategies particularly at livestock-wildlife interface.
小反刍兽疫病毒（PPRV）感染范围广泛，包括多种家养和野生反刍动物，偶尔也会感染骆驼、牛和猪等不常见宿主。鉴于其宿主谱广泛，且在多个发展中国家流行，因此必须阐明病毒的进化见解，以了解其动态病理学和对不同宿主的选择。为此，我们构建并进行了计算机分析了来自野生和不常见宿主的所有可用（n=37）PPRV 序列数据集。与来自同一地理区域的家养小反刍动物毒株相比，来自野生和不常见宿主的 PPRV 序列的系统基因组和残基分析显示，这些毒株之间存在密切关系。研究序列和推断的残基之间没有明显差异，这表明这些是宿主因素，可能在其对 PPRV 感染的易感性、免疫反应、发病机制、排泄模式以及潜在的临床症状或对临床疾病的抵抗力方面发挥作用。总之，比较分析增强了我们对野生和不常见宿主中 PPRV 的分子流行病学的理解，以便在牲畜-野生动物界面采取适当的干预策略。
