Centro Nacional de Microbiología/ISCIII, Madrid, Spain.
Adv Exp Med Biol. 2019;1215:45-68. doi: 10.1007/978-3-030-14741-9_3.
To initiate infection, non-enveloped viruses must recognize a target cell and penetrate the cell membrane by pore formation or membrane lysis. Rotaviruses are non-enveloped dsRNA viruses that infect the mature intestinal epithelium. They are major etiologic agents of diarrheal disease in human infants, as well as in young individuals of various avian and mammalian species. Rotavirus entry into the cell is a complex multistep process initiated by the interaction of the tip of the viral spike with glycan ligands at the cell surface, and driven by conformational changes of the proteins present in the outer protein capsid, the viral machinery for entry. This review feeds on the abundant structural information produced for rotavirus during the past 30 years and focuses on the structure and the dynamics of the rotavirus entry machinery. We survey the current models for rotavirus entry into cells.
为了引发感染，无包膜病毒必须识别靶细胞，并通过形成孔或膜裂解穿透细胞膜。轮状病毒是感染成熟肠道上皮细胞的无包膜双链 RNA 病毒。它们是引起人类婴儿以及各种禽类和哺乳动物幼崽腹泻病的主要病原体。轮状病毒进入细胞是一个复杂的多步骤过程，由病毒刺突的尖端与细胞表面的聚糖配体相互作用启动，并由存在于外壳蛋白中的蛋白质的构象变化驱动，这些蛋白质是进入病毒机制。这篇综述利用了过去 30 年来针对轮状病毒产生的丰富结构信息，重点介绍了轮状病毒进入细胞的机制的结构和动态。我们调查了轮状病毒进入细胞的现行模型。
