A.M. Butlerov Institute of Chemistry, Kazan Federal University, Kazan, Russia.
Zavoisky Physical-Technical Institute of FRC Kazan Scientific Center of RAS, Kazan, Russia.
J Pept Sci. 2019 Aug;25(8):e3177. doi: 10.1002/psc.3177.
Thermal treatment of short-chain oligopeptides is able to initiate the process of their self-assembly with the formation of organic nanostructures with unique properties. On the other hand, heating can lead to a chemical reaction with the formation of new substances with specific properties and ability to form structures with different morphology. Therefore, in order to have a desired process, researcher needs to find its temperature range. In the present work, cyclization of -isoleucyl- -alanine dipeptide in the solid state upon heating was studied. Kinetic parameters of this reaction were estimated within the approaches of the nonisothermal kinetics. The correlation between side chain structure of dipeptides and temperature of their cyclization in the solid state was found for the first time. This correlation may be used to predict the temperature, at which dipeptide self-assembly changes to chemical reaction. The differences in self-assembly of linear and cyclic dipeptides were demonstrated using atomic force microscopy. The effect of dipeptide concentration in a source solution and an organic solvent used on self-assembly of dipeptides was shown. The new information obtained on the thermal properties and self-assembly of linear and cyclic forms of -isoleucyl- -alanine may be useful for the design of new nanomaterials based on oligopeptides, as well as for the synthesis of cyclic oligopeptides.
短链寡肽的热处理能够引发它们的自组装过程，形成具有独特性质的有机纳米结构。另一方面，加热会导致化学反应，形成具有特定性质和形成不同形态结构能力的新物质。因此，为了获得所需的过程，研究人员需要找到其温度范围。在本工作中，研究了加热时固态 -异亮氨酰- -丙氨酸二肽的环化反应。通过非等温热力学方法估算了该反应的动力学参数。首次发现了二肽侧链结构与固态环化温度之间的相关性。该相关性可用于预测二肽自组装转变为化学反应的温度。使用原子力显微镜证明了线性和环状二肽的自组装的差异。展示了源溶液中二肽浓度和所用有机溶剂对二肽自组装的影响。获得的关于线性和环状 -异亮氨酰- -丙氨酸形式的热性质和自组装的新信息可能对基于寡肽的新型纳米材料的设计以及环状寡肽的合成有用。
