（NZB×NZW）F1小鼠中编码双链DNA抗体重链可变区的S107（T15）VH11种系基因的体细胞多样化。
Somatic diversification of the S107 (T15) VH11 germ-line gene that encodes the heavy-chain variable region of antibodies to double-stranded DNA in (NZB x NZW)F1 mice.
作者信息
Behar S M, Scharff M D
机构信息
Department of Cell Biology, Irvington House Institute for Medical Research, Albert Einstein College of Medicine, Bronx, NY 10461.
出版信息
Proc Natl Acad Sci U S A. 1988 Jun;85(11):3970-4. doi: 10.1073/pnas.85.11.3970.
Abstract
A variety of studies suggest that members of the S107 (T15) heavy-chain variable-region gene family contribute to the autoimmune response of mice and humans to DNA. To identify the germ-line gene(s) involved and the degree of somatic diversification that occurs in such autoantibodies, we determined the mRNA sequence of the heavy and light chains of a group of monoclonal anti-DNA antibodies encoded by the S107 VH11 germ-line gene in (NZB x NZW)F1 mice. We also cloned and sequenced the VH11 germ-line gene of the NZB and NZW parental strains. The VH11 coding sequences of the two strains were identical. Comparison with this heavy-chain germ-line sequence showed that the variable regions of the monoclonal antibodies had undergone considerable somatic diversification.
摘要
多项研究表明，S107（T15）重链可变区基因家族的成员参与了小鼠和人类对DNA的自身免疫反应。为了确定所涉及的种系基因以及此类自身抗体中发生的体细胞多样化程度，我们测定了（NZB×NZW）F1小鼠中由S107 VH11种系基因编码的一组单克隆抗DNA抗体的重链和轻链的mRNA序列。我们还克隆并测序了NZB和NZW亲本菌株的VH11种系基因。两个菌株的VH11编码序列相同。与该重链种系序列的比较表明，单克隆抗体的可变区经历了相当程度的体细胞多样化。
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