Institute of Edible Fungi, Shanghai Academy of Agriculture Sciences, National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai 201403, PR China.
Institute of Edible Fungi, Shanghai Academy of Agriculture Sciences, National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai 201403, PR China; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Carbohydr Polym. 2019 Oct 15;222:114996. doi: 10.1016/j.carbpol.2019.114996. Epub 2019 Jun 19.
H6PC20, a water-soluble β-D-glucan, with a molecular mass of 2390 kDa (HPSEC-MALLS-RI), was obtained by extracting the fresh fruiting bodies of Hericium erinaceus at mature stage with hot water, freeze-thawing, precipitating and further rinsing with 20% (v/v) of ethanol. The primary structure of H6PC20 was proposed as a β-(1→3)-linked-D-glucan with a (1→6)-β-linked-D- glucopyranosyl side-branching unit on every third residue by using methylation analysis, H and C NMR spectroscopy, including COSY, TOCSY, HSQC and HMBC. The ρ value (R/R) and the exponent (α) of [η] = k M of H6PC20 were calculated as 1.86 and 0.54, respectively, indicating flexible random coil conformation of H6PC20 in aqueous solution. H6PC20 could induce the proliferation of lymphocytes and enhance the expression of inflammatory cytokines IL-6, TNF-α and IL-1β secreted by THP-1 macrophages. The present study indicated that the macromolecular β-glucan H6PC20 possessed potent immune-enhancing bioactivities and could be a potential ingredient for healthy food or medicine development.
H6PC20 是一种水溶性 β-D-葡聚糖，其相对分子质量为 2390 kDa（HPSEC-MALLS-RI），通过热水提取成熟期猴头菇的新鲜子实体，经过反复冻融、沉淀，并用 20%（v/v）乙醇进一步冲洗得到。通过甲基化分析、氢谱和碳谱（包括 COSY、TOCSY、HSQC 和 HMBC）等波谱分析，提出 H6PC20 的一级结构为β-（1→3）-D-葡聚糖，每第三个残基上有（1→6）-β-D-葡萄糖吡喃基侧链分支单元。H6PC20 的 ρ 值（R/R）和[η] = kM 的指数（α）分别计算为 1.86 和 0.54，表明 H6PC20 在水溶液中呈现出灵活的无规卷曲构象。H6PC20 可以诱导淋巴细胞增殖，并增强 THP-1 巨噬细胞分泌的炎症细胞因子 IL-6、TNF-α 和 IL-1β 的表达。本研究表明，该高分子量β-葡聚糖 H6PC20 具有很强的免疫增强活性，可作为开发健康食品或药物的潜在成分。
