潜在感染传播源：在各种环境样本中的检测。
Potential transmission sources of infection: detection of in various environmental samples.
作者信息
Farhadkhani Marzieh, Nikaeen Mahnaz, Hassanzadeh Akbar, Nikmanesh Bahram
机构信息
1Student Research Committee, School of Health, Isfahan University of Medical Sciences, Isfahan, Iran.
2Department of Environmental Health Engineering, School of Health, Isfahan University of Medical Sciences, Hezar Jerib Avenue, Isfahan, Iran.
出版信息
J Environ Health Sci Eng. 2019 Jan 3;17(1):129-134. doi: 10.1007/s40201-018-00333-y. eCollection 2019 Jun.
PURPOSE
is one of the most common human infectious agents which may be transmitted via water. This study was designed to test presence via molecular methods in various aquatic environments as well as sewage sludge (SS) to understand the role of these environments in the pathogen's transmission.
METHODS
specific primers for the 16S rRNA and A genes of were used in a nested and semi-nested PCR, respectively. Detection sensitivity of in environmental samples by semi-nested PCR was also compared with real-time PCR. Analysis of fecal coliforms (FC) as pollution indicator bacteria was also performed.
RESULTS
16S rRNA gene was detected in 36% (14/39) of wastewater samples and 8% (2/25) of water samples, while amplification of A gene yielded only two positive result. None of the SS samples were positive for and real-time PCR could not identify in any of the samples. The results showed no correlation between the presence of and FC.
CONCLUSIONS
Our result revealed the widespread presence of in wastewater samples which indicates wastewater may be a source for dissemination and transmission of infection. Further research is needed to determine the risk of in wastewater reuse for irrigation of crops.
目的
是最常见的人类传染因子之一，可通过水传播。本研究旨在通过分子方法检测各种水生环境以及污水污泥（SS）中 的存在情况，以了解这些环境在病原体传播中的作用。
方法
分别使用针对 的16S rRNA和A基因的特异性引物进行巢式和半巢式PCR。还将半巢式PCR检测环境样品中 的灵敏度与实时PCR进行了比较。同时也对作为污染指示菌的粪大肠菌群（FC）进行了分析。
结果
在36%（14/39）的废水样品和8%（2/25）的水样中检测到16S rRNA基因，而A基因的扩增仅产生两个阳性结果。所有SS样品均未检测到 呈阳性，实时PCR在任何样品中均未鉴定出 。结果表明 的存在与FC之间无相关性。
结论
我们的结果揭示了废水中 广泛存在，这表明废水可能是 感染传播和扩散的一个来源。需要进一步研究以确定废水用于灌溉作物时 的风险。
Zotero 插件