足月孕妇阴道酵母菌内的[具体物质]及其毒力相关基因型的细胞内存在情况 。 （你原文中“Intracellular Presence of and”这里少了个具体物质，我只能按这个形式翻译了）
Intracellular Presence of and Its Virulence-Associated Genotypes within the Vaginal Yeast of Term Pregnant Women.
作者信息
Sánchez-Alonzo Kimberly, Matamala-Valdés Lillian, Parra-Sepúlveda Cristian, Bernasconi Humberto, Campos Víctor L, Smith Carlos T, Sáez Katia, García-Cancino Apolinaria
机构信息
Laboratory of Bacterial Pathogenicity, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepcion, Concepción 4070386, Chile.
Department of Obstetrics, Faculty of Medicine, Universidad de Concepción, Concepción 4070386, Chile.
出版信息
Microorganisms. 2021 Jan 8;9(1):131. doi: 10.3390/microorganisms9010131.
BACKGROUND
transmission routes are not entirely elucidated. Since yeasts are postulated to transmit this pathogen, this study aimed to detect and genotype intracellular harbored within vaginal yeast cells.
METHODS
A questionnaire was used to determine risk factors of infection. Samples were seeded on Sabouraud Dextrose Agar and horse blood-supplemented Columbia agar. Isolated yeasts were identified using and observed by optical microscopy searching for intra-yeast . Total yeast DNA, from one random sample, was extracted to search for virulence genes by PCR and bacterial identification by sequencing.
RESULTS
43% of samples contained yeasts, mainly (91%). Microscopy detected bacteria such as bodies and anti- antibodies binding particles in 50% of the isolated yeasts. Total DNA extracted showed that 50% of the isolated yeasts were positive for 16S rDNA and the sequence showed 99.8% similarity with In total, 32% of DNA positive samples were . No relationship was observed between possible infection risk factors and vaginal yeasts harboring this bacterium.
CONCLUSION
having virulent genotypes were detected within vaginal yeasts constituting a risk for vertical transmission of this pathogen.
背景
传播途径尚未完全阐明。由于推测酵母会传播这种病原体，本研究旨在检测阴道酵母细胞内携带的病原体并进行基因分型。
方法
使用问卷调查确定感染的风险因素。将样本接种在沙氏葡萄糖琼脂和补充马血的哥伦比亚琼脂上。使用[具体方法]鉴定分离出的酵母，并通过光学显微镜观察寻找酵母内的[具体物质]。从一个随机样本中提取总酵母DNA，通过聚合酶链反应（PCR）寻找毒力基因，并通过测序进行细菌鉴定。
结果
43%的样本含有酵母，主要是[具体酵母种类]（91%）。显微镜检查在50%的分离酵母中检测到如[具体细菌结构]和抗[具体抗体]结合颗粒等细菌。提取的总DNA显示，50%的分离酵母16S核糖体DNA（rDNA）呈阳性，序列显示与[具体细菌]有99.8%的相似性。总体而言，32%的DNA阳性样本为[具体细菌]。未观察到可能的[具体感染名称]感染风险因素与携带这种细菌的阴道酵母之间存在关联。
结论
在阴道酵母中检测到具有毒力的基因型，这构成了该病原体垂直传播的风险。
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