Department of General Surgery, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Pudong, Shanghai 201399, P.R. China.
Oncol Rep. 2019 Sep;42(3):1141-1148. doi: 10.3892/or.2019.7220. Epub 2019 Jul 4.
Interleukin‑17F (IL‑17F) is a member of the IL‑17 family of proteins. Previous studies concerning IL‑17F have mainly focused on its proinflammatory responses, whereas the present study explored its role as an oncogene in colorectal cancer (CRC). The present study investigated the expression of IL‑17F in 69 patients with CRC. IL‑17F was significantly overexpressed in tumor mucosa compared with that in the paired non‑tumor mucosa. Furthermore, several studies from Gene Expression Omnibus (GEO) databases were analyzed to assess the association between IL‑17F and overall survival and relapse‑free survival time. Recombinant human IL‑17F protein (rhIL‑17F) and anti‑IL‑17F antibody were used to study the effect of IL‑17F on the CRC cell line HCT‑116 in vitro. According to the results of Transwell and wound healing assays, rhIL‑17F promoted HCT‑116 cell migration and invasion which was mediated by inducing epithelial‑mesenchymal transition (EMT), whereas anti‑IL‑17F inhibited EMT.
白细胞介素 17F（IL-17F）是白细胞介素 17 家族蛋白的成员。先前关于 IL-17F 的研究主要集中在其促炎反应上，而本研究则探讨了其在结直肠癌（CRC）中的致癌基因作用。本研究调查了 69 例 CRC 患者中 IL-17F 的表达。与配对的非肿瘤黏膜相比，肿瘤黏膜中 IL-17F 的表达明显上调。此外，还对来自基因表达综合数据库（GEO）的几项研究进行了分析，以评估 IL-17F 与总生存时间和无复发生存时间之间的关联。使用重组人白细胞介素 17F 蛋白（rhIL-17F）和抗白细胞介素 17F 抗体来研究 IL-17F 对体外 CRC 细胞系 HCT-116 的影响。根据 Transwell 和划痕愈合试验的结果，rhIL-17F 促进 HCT-116 细胞迁移和侵袭，这是通过诱导上皮-间充质转化（EMT）介导的，而抗 IL-17F 则抑制 EMT。
