Department of Biomedical Sciences and Pathobiology, Virginia Tech, VA-MD Regional College of Veterinary Medicine, Blacksburg, VA, USA.
Department of Biomedical Sciences and Pathobiology, Virginia Tech, VA-MD Regional College of Veterinary Medicine, Blacksburg, VA, USA.
Virology. 2019 Sep;535:227-231. doi: 10.1016/j.virol.2019.07.013. Epub 2019 Jul 14.
Mayaro virus (MAYV; family Togaviridae, genus Alphavirus) is an emerging global threat that can cause severe clinical manifestations similar to Zika, dengue, and chikungunya viruses. Currently, there is a lack of molecular tools to enable a better understanding of the transmission and pathogenesis of MAYV. Here, we detail the development and characterization of infectious clones of two strains of MAYV that produce infectious virus and replicate in mammalian and mosquito cells similarly to wild-type virus. Additionally, clone-derived viruses produced identical infection rates and phenotypes in CD-1 mice compared to the parental strains. This infectious clone system will provide a resource to the research community to analyze MAYV genetic determinants of virulence, determine vector competence, and develop vaccines.
马亚罗病毒（MAYV；黄病毒科，甲病毒属）是一种新出现的全球威胁，可引起类似于寨卡、登革热和基孔肯雅热病毒的严重临床症状。目前，缺乏分子工具来更好地了解 MAYV 的传播和发病机制。在这里，我们详细介绍了两种 MAYV 株的传染性克隆的开发和鉴定，这些克隆能够产生感染性病毒，并在哺乳动物和蚊子细胞中与野生型病毒类似地复制。此外，与亲本株相比，源自克隆的病毒在 CD-1 小鼠中产生了相同的感染率和表型。这个传染性克隆系统将为研究界提供一种资源，用于分析 MAYV 的毒力遗传决定因素、确定媒介的感染力，并开发疫苗。
