一种含纳米 CaF 的正畸水泥，具有抗菌和再矿化能力，可对抗釉质白斑病损。

A nano-CaF-containing orthodontic cement with antibacterial and remineralization capabilities to combat enamel white spot lesions.

机构信息

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Dept. of Orthodontic and Pediatric Dentistry, West China Hospital of Stomatology, Sichuan University, China; Department of Advanced Oral Sciences and Therapeutics, School of Dentistry, University of Maryland, Baltimore, MD 21021, USA.

Department of Advanced Oral Sciences and Therapeutics, School of Dentistry, University of Maryland, Baltimore, MD 21021, USA; Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases, Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, Department of General Dentistry, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shaanxi 710004, China.

出版信息

J Dent. 2019 Oct;89:103172. doi: 10.1016/j.jdent.2019.07.010. Epub 2019 Jul 18.

DOI:10.1016/j.jdent.2019.07.010

PMID:31326528

Abstract

OBJECTIVES

The objectives of this study were to develop a resin-modified glass ionomer containing nanoparticles of calcium fluoride (nCaF) and dimethylaminohexadecyl methacrylate (DMAHDM) for the first time and investigate the antibacterial and remineralization properties.

METHODS

nCaF was synthesized using a spray-drying method and characterized using a transmission electron microscope. Twenty weight percentage (wt%) nCaF and 3 wt% DMAHDM were incorporated into a RMGI (GC Ortho LC). Enamel shear bond strength (SBS) and cytotoxicity were determined. Fluoride (F) and calcium (Ca) ion releases were assessed. Biofilm live/dead staining, metabolic activity, polysaccharide and lactic production, and colony-forming units (CFU) were evaluated. The remineralization ability was determined by measuring the effects of cements on enamel surface hardness and lesion depth.

RESULTS

Incorporating 20 wt% nCaF and 3 wt% DMAHDM did not compromise the SBS (p > 0.1). The decrease of pH from 7.0 to 4.0 significantly increased the F and Ca ion releases. The new cement greatly reduced the metabolic activity, polysaccharide and lactic acid productions, and lowered the biofilm CFU by 3 log, compared to commercial control (p < 0.05). The new cement increased the enamel hardness by 56% and decreased the lesion depth by 43%, compared to control (p < 0.05). The cell viability at 7 days against the new cement extracts was 82.2% of that of the negative control in culture medium without any extracts.

CONCLUSIONS

The novel orthodontic cement containing nCaF and DMAHDM achieved much stronger antibacterial and remineralization capabilities and greater enamel hardness than the commercial control did, without compromising the orthodontic bracket-enamel SBS and biocompatibility.

CLINICAL SIGNIFICANCE

The novel bioactive and nanostructured orthodontic cement is promising to inhibit enamel demineralization, white spot lesions and caries in orthodontic treatments.

摘要

目的

本研究首次开发了一种含有纳米氟化钙（nCaF）和二甲基氨基己基甲基丙烯酸酯（DMAHDM）的树脂改性玻璃离子体，并研究其抗菌和再矿化性能。

方法

采用喷雾干燥法合成 nCaF，并用透射电子显微镜进行表征。将 20wt%的 nCaF 和 3wt%的 DMAHDM 掺入 RMGI（GC Ortho LC）中。测定釉质剪切粘结强度（SBS）和细胞毒性。评估氟（F）和钙（Ca）离子释放。通过生物膜活/死染色、代谢活性、多糖和乳酸产生以及菌落形成单位（CFU）评估。通过测量粘结剂对釉质表面硬度和病变深度的影响来确定再矿化能力。

结果

掺入 20wt%的 nCaF 和 3wt%的 DMAHDM 不会影响 SBS（p>0.1）。pH 值从 7.0 降至 4.0 时，F 和 Ca 离子释放量显著增加。与商业对照相比，新型水泥显著降低了代谢活性、多糖和乳酸的产生，使生物膜 CFU 降低了 3 个对数级（p<0.05）。与对照相比，新型水泥使釉质硬度提高了 56%，病变深度降低了 43%（p<0.05）。新水泥提取物培养 7 天后的细胞活力为无提取物培养基中阴性对照的 82.2%。