Department of Animal Sciences, Pennsylvania State University, University Park, PA, USA.
The Huck Institutes, Pennsylvania State University, University Park, PA, USA.
Sci Adv. 2019 Jul 17;5(7):eaax4899. doi: 10.1126/sciadv.aax4899. eCollection 2019 Jul.
Bovine tuberculosis (bTB) is a major zoonotic disease of cattle that is endemic in much of the world, limiting livestock productivity and representing a global public health threat. Because the standard tuberculin skin test precludes implementation of Bacille Calmette-Guérin (BCG) vaccine-based control programs, we here developed and evaluated a novel peptide-based defined antigen skin test (DST) to diagnose bTB and to differentiate infected from vaccinated animals (DIVA). The results, in laboratory assays and in experimentally or naturally infected animals, demonstrate that the peptide-based DST provides DIVA capability and equal or superior performance over the extant standard tuberculin surveillance test. Together with the ease of chemical synthesis, quality control, and lower burden for regulatory approval compared with recombinant antigens, the results of our studies show that the DST considerably improves a century-old standard and enables the development and implementation of critically needed surveillance and vaccination programs to accelerate bTB control.
牛结核病（bTB）是一种主要的动物传染病，在世界上许多地方流行，限制了牲畜的生产力，并构成了全球公共卫生威胁。由于标准结核菌素皮试排除了卡介苗（BCG）疫苗控制计划的实施，我们在这里开发并评估了一种新的基于肽的定义抗原皮试（DST），以诊断 bTB 并区分感染动物和接种疫苗动物（DIVA）。实验室检测以及实验性或自然感染动物的结果表明，基于肽的 DST 提供了 DIVA 能力，并且与现有的标准结核菌素监测测试具有同等或更高的性能。与重组抗原相比，基于肽的 DST 具有化学合成简便、质量控制容易以及监管批准负担较小的优势，我们的研究结果表明，该 DST 大大改进了一个世纪以来的标准，并能够制定和实施急需的监测和疫苗接种计划，以加速 bTB 控制。
