a Department of Urology , Affiliated Hospital of North Sichuan Medical College , Nanchong , Sichuan , China.
b Department of Dermatovenereology , Affiliated Hospital of North Sichuan Medical College , Nanchong , Sichuan , China.
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):512-523. doi: 10.1080/21691401.2018.1560305.
In recent times, Gold nanoparticles (AuNPs) synthesized from plant extracts and their anticancer activity have attracted significant attention. We report the green approach for the synthesis of AuNPs using extract from Abies spectabilis plant. In this study, the reaction parameters were optimized to control the size of the nanoparticle, which was confirmed by Transmission Electron microscopy (TEM). Various characterization technique such as SAED pattern, UV visible spectroscopy, EDX, FTIR, and AFM were employed to analyze the synthesized AuNPs obtained from A. spectabilis plant extract. Furthermore, we investigated the anticancer activities using T24 cell lines. Interestingly, the results of extensive screening on the applications of newly synthesized AuNPs were tested for their cytotoxicity effects on anticancer activity against T24 cells by MTT assay. The cell apoptosis was studied using TUNEL, DAPI, caspase activity, cell invasion and migration. Nanoparticles at different concentrations ranging from 1 to 25 μg/ml exhibited a dose dependent cytotoxicity for 24 h. Condensation and DNA fragmentation are characteristic of apoptosis by DAPI, TUNEL staining, and the significant up regulations of Beclin-1, Bax and caspase 3, whereas the expressions of anti-apoptotic Bcl-2 and Bid were down regulated. However, this study, therefore attempts to report the synthesis, characterization, and anticancer activity of gold nanoparticles of A. spectabilis plant extract beneficial for cancer therapeutics.
近年来，植物提取物合成的金纳米粒子（AuNPs）及其抗癌活性引起了广泛关注。我们报告了一种使用冷杉属植物提取物合成 AuNPs 的绿色方法。在这项研究中，通过透射电子显微镜（TEM）确认了优化反应参数以控制纳米粒子尺寸。采用 SAED 图案、紫外可见光谱、EDX、FTIR 和 AFM 等各种表征技术对从冷杉属植物提取物中获得的合成 AuNPs 进行了分析。此外，我们还使用 T24 细胞系研究了抗癌活性。有趣的是，对新合成的 AuNPs 的广泛应用进行了广泛的筛选，通过 MTT 测定法测试了它们对 T24 细胞的抗癌活性的细胞毒性作用。通过 TUNEL、DAPI、半胱氨酸天冬氨酸蛋白酶活性、细胞侵袭和迁移研究细胞凋亡。在 1 至 25μg/ml 的不同浓度下，纳米粒子表现出 24 小时的剂量依赖性细胞毒性。DAPI、TUNEL 染色和 Beclin-1、Bax 和半胱氨酸天冬氨酸蛋白酶 3 的显著上调表明细胞凋亡的特征是核浓缩和 DNA 片段化，而抗凋亡 Bcl-2 和 Bid 的表达下调。然而，本研究因此试图报告冷杉属植物提取物合成、表征和抗癌活性的 AuNPs，有益于癌症治疗。
