Benincasa Cinzia, La Torre Chiara, Plastina Pierluigi, Fazio Alessia, Perri Enzo, Caroleo Maria Cristina, Gallelli Luca, Cannataro Roberto, Cione Erika
CREA-Research Centre for Olive, Citrus and Tree Fruit, C.da Li Rocchi, 87036 Rende (CS), Italy.
Department of Pharmacy, Health and Nutrition Sciences, University of Calabria, Via Pietro Bucci, 87036 Arcavacata di Rende (CS), Italy.
Antioxidants (Basel). 2019 Jul 20;8(7):233. doi: 10.3390/antiox8070233.
Recently, we identified hydroxytyrosyl oleate (HtyOle) in the by-products of olive oil, pomace and olive mill waste water (OMWW). Herein, we report that HtyOle is more accurately quantified by extracting the phenolic fraction from both matrices by using aqueous methanol (80%). By applying this method, HtyOle was also detected in extra virgin olive oil (EVOO). Since olive oil is used in the preparation of many cosmetic formulations, we explored the antioxidant capacity of HtyOle in human keratinocytes. Formation of reactive oxygen species (ROS) and malondialdehyde (MDA), as well as activity of Glutathione-S-transferase (GST) and superoxide dismutase (SOD) were decreased by HtyOle. In addition to that, microRNAs (miRs) involved in both redox status balance and skin regeneration potential were also tested. The following miRs, hsa-miR-21 and hsa-miR-29a, were increased while has-miR-34a was not affected by HtyOle.
最近，我们在橄榄油副产品、果渣和橄榄油厂废水（OMWW）中鉴定出了羟基酪醇油酸酯（HtyOle）。在此，我们报告称，通过使用80%的甲醇水溶液从两种基质中提取酚类部分，可以更准确地定量HtyOle。应用该方法，在特级初榨橄榄油（EVOO）中也检测到了HtyOle。由于橄榄油用于制备许多化妆品配方，我们研究了HtyOle在人角质形成细胞中的抗氧化能力。HtyOle降低了活性氧（ROS）和丙二醛（MDA）的形成，以及谷胱甘肽-S-转移酶（GST）和超氧化物歧化酶（SOD）的活性。此外，还测试了参与氧化还原状态平衡和皮肤再生潜能的微小RNA（miRs）。以下miRs，即hsa-miR-21和hsa-miR-29a增加，而has-miR-34a不受HtyOle影响。
