Xue Yanbin, Ma Jun, He Yehua, Yu Sanmiao, Lin Zhen, Xiong Yingyuan, Rafique Fatima, Jiang Fuxing, Sun Lingxia, Ma Mingdong, Zhou Yujue, Li Xi, Huang Zhuo
College of Landscape Architecture, Sichuan Agricultural University, Chengdu, Sichuan, China.
Horticultural Biotechnology College of South China Agricultural University, South China Agricultural University, Guangzhou, Guangdong, China.
PeerJ. 2019 Jul 10;7:e7261. doi: 10.7717/peerj.7261. eCollection 2019.
var. has high ornamental value due to its chimeric leaves. However, the chimeric trait is very unstable in red pineapple plants, and transcriptional variation between the two types of cells (white/green cells) and the molecular mechanism responsible for their albino phenotype remain poorly understood.
Comparative transcriptomic and proteomic analyses of the white parts (Whs) and green parts (Grs) of chimeric leaves were performed.
In total, 1,685 differentially expressed genes (DEGs) (712 upregulated and 973 downregulated) and 1,813 differentially abundant proteins (DAPs) (1,018 with low abundance and 795 with high abundance) were identified. Based on Gene Ontology (Go) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses, the DEGs were mostly involved in carbon fixation in photosynthetic organisms, porphyrin and chlorophyll metabolism and oxidative phosphorylation, while proteomic analysis revealed that DAPs were mostly related to ribosomes, photosynthesis, photosynthesis antennas, and porphyrin and chlorophyll metabolism. Combined analysis showed increased mRNA levels but low abundance of nine proteins level in Whs /Grs related to photosynthetic pigment and photosynthesis. Transcriptional changes, posttranscriptional regulation and translational alterations of key enzymes involved in chlorophyll biosynthesis and photosynthesis may play important roles in the albino parts of chimeric leaves.
var. 因其嵌合叶具有较高的观赏价值。然而，嵌合性状在红菠萝植株中非常不稳定，两种类型的细胞（白色/绿色细胞）之间的转录变异以及导致其白化表型的分子机制仍知之甚少。
对嵌合叶的白色部分（Whs）和绿色部分（Grs）进行了比较转录组学和蛋白质组学分析。
共鉴定出1685个差异表达基因（DEGs）（712个上调和973个下调）和1813个差异丰富蛋白质（DAPs）（1018个低丰度和795个高丰度）。基于基因本体论（Go）和京都基因与基因组百科全书（KEGG）通路富集分析，DEGs主要参与光合生物中的碳固定、卟啉和叶绿素代谢以及氧化磷酸化，而蛋白质组学分析表明DAPs主要与核糖体、光合作用、光合天线以及卟啉和叶绿素代谢有关。联合分析显示，与光合色素和光合作用相关的9种蛋白质在Whs /Grs中的mRNA水平升高但丰度较低。参与叶绿素生物合成和光合作用的关键酶的转录变化、转录后调控和翻译改变可能在嵌合叶的白化部分中起重要作用。
