Gargano Valeria, Sciortino Sonia, Gambino Delia, Costa Antonella, Agozzino Vincenzo, Reale Stefano, Alduina Rosa, Vicari Domenico
Istituto Zooprofilattico Sperimentale della Sicilia, 90129 Palermo, Italy.
Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, 90028 Palermo, Italy.
Antibiotics (Basel). 2021 Jul 2;10(7):809. doi: 10.3390/antibiotics10070809.
spp. is among the leading causes of foodborne infections in humans and a large number of animals. spp. is a pathogen involved in the dissemination of antimicrobial resistance because it can accumulate antibiotic resistance genes (ARGs). In this study, the antibiotic resistance profile to 15 antibiotics, belonging to six different classes, of 60 strains of spp. collected from pets, farm animals, wildlife, and food in Sicily (Italy) was investigated by the Kirby-Bauer method. Given that almost 33.3% of the spp. strains were resistant to tetracycline, Real-Time PCR analysis was applied on all the 60 strains to detect the presence of eight selected resistance genes. Besides, the presence of the gene, related to the horizontal gene transfer among bacteria, was also investigated in all the strains by Real-Time PCR analysis. Our data showed that 56% of the isolated strains harbored one or more resistance genes and that these strains were most frequently isolated from animals living in close contact with humans. Concerning , 17 strains (28.3%) harbored this genetic element and eight of these simultaneously contained genes. The results of this study highlight the importance of using a molecular approach to detect resistance genetic determinants, whose spread can increase the diffusion of multidrug-resistant strains. Besides, the study of zoonotic bacteria such as spp. which significantly contribute to ARGs dissemination should always follow a One Health approach that considers the health of humans, animals, and the environment to be closely related.
某菌属是人类和大量动物食源性感染的主要原因之一。该菌属是一种与抗菌药物耐药性传播有关的病原体，因为它可以积累抗生素耐药基因（ARGs）。在本研究中，采用 Kirby-Bauer 方法对从意大利西西里岛的宠物、农场动物、野生动物和食物中收集的 60 株某菌属菌株对属于六个不同类别的 15 种抗生素的耐药谱进行了调查。鉴于近 33.3%的某菌属菌株对四环素耐药，对所有 60 株菌株进行了实时荧光定量 PCR 分析，以检测八个选定的耐药基因的存在。此外，还通过实时荧光定量 PCR 分析在所有菌株中研究了与细菌间水平基因转移相关的该基因的存在情况。我们的数据显示，56%的分离菌株携带一个或多个耐药基因，并且这些菌株最常从与人类密切接触的动物中分离出来。关于该基因，17 株（28.3%）携带了这种遗传元件，其中八株同时含有耐药基因。本研究结果强调了使用分子方法检测耐药遗传决定因素的重要性，其传播可增加多重耐药菌株的扩散。此外，对诸如某菌属这种显著促进 ARGs 传播的人畜共患病细菌的研究应始终遵循“同一健康”方法，该方法认为人类、动物和环境的健康密切相关。
