School of Textile Science and Engineering , Xi'an Polytechnic University , Xi'an 710048 , P. R. China.
Key Laboratory of Textile Science & Technology, Ministry of Education, College of Textiles , Donghua University , Shanghai 201620 , P. R. China.
ACS Appl Mater Interfaces. 2019 Aug 14;11(32):28740-28751. doi: 10.1021/acsami.9b10379. Epub 2019 Aug 5.
Electrospinning provides a simple and convenient method to fabricate nanofibrous meshes. However, the nanofiber productivity is often limited to the laboratory scale, which cannot satisfy the requirements of practical application. In this study, we developed a novel needleless electrospinning spinneret based on a double-ring slit to fabricate drug-loaded nanofibrous meshes. In contrast to the conventional single-needle electrospinning spinneret, our needless spinneret can significantly improve nanofiber productivity due to the simultaneous formation of multiple jets during electrospinning. Curcumin-loaded poly(l-lactic acid) (PLLA) nanofiber meshes with various concentrations and on the large scale were manufactured by employing our developed needleless spinneret-based electrospinning device. We systematically investigated the drug release behaviors, antioxidant properties, anti-inflammatory attributes, and cytotoxicity of the curcumin-loaded PLLA nanofibrous meshes. Furthermore, a bilayer nanofibrous composite mesh was successfully generated by electrospinning curcumin-loaded PLLA solution and diclofenac sodium loaded poly(ethylene oxide) solution in a predetermined time sequence, which revealed potent antibacterial properties. Subsequently, novel mucoadhesive patches were assembled by combining the bilayer composite nanofibrous meshes with (hydroxypropyl)methyl cellulose based mucoadhesive film. The multilayered mucoadhesive patch has excellent adhesion properties on the porcine buccal mucosa. Overall, our double-ring slit spinneret can provide a novel method to rapidly produce large-scale drug-loaded nanofibrous meshes to fabricate mucoadhesive patches. The multiple-layered mucoadhesive patches enable the incorporation of multiple drugs with different targets of action, such as analgesic, anti-inflammatory, and antimicrobial compounds, for mouth ulcer or other oral disease treatments.
静电纺丝提供了一种简单便捷的方法来制备纳米纤维网。然而，纳米纤维的生产率通常限于实验室规模，无法满足实际应用的要求。在这项研究中，我们开发了一种基于双环狭缝的新型无针静电纺丝喷丝头，用于制备载药纳米纤维网。与传统的单针静电纺丝喷丝头相比，我们的无针喷丝头可以通过在静电纺丝过程中同时形成多个射流，显著提高纳米纤维的生产率。通过使用我们开发的基于无针喷丝头的静电纺丝装置，制造了具有各种浓度和大规模的载姜黄素聚（L-丙交酯）（PLLA）纳米纤维网。我们系统地研究了载姜黄素 PLLA 纳米纤维网的药物释放行为、抗氧化性能、抗炎特性和细胞毒性。此外，通过在预定的时间序列中静电纺丝载姜黄素的 PLLA 溶液和载双氯芬酸钠的聚（氧化乙烯）溶液，成功生成了双层纳米纤维复合网，显示出强大的抗菌性能。随后，通过将双层复合纳米纤维网与基于（羟丙基）甲基纤维素的粘膜粘附膜结合，组装了新型粘膜粘附贴片。多层粘膜粘附贴片在猪颊粘膜上具有出色的粘附性能。总体而言，我们的双环狭缝喷丝头可以提供一种新的方法来快速生产大规模载药纳米纤维网，以制造粘膜粘附贴片。多层粘膜粘附贴片可以将具有不同作用靶点的多种药物（如镇痛、抗炎和抗菌化合物）结合在一起，用于口腔溃疡或其他口腔疾病的治疗。
