State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, 102206, P. R. China.
Shanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, P. R. China.
J Microbiol. 2022 Feb;60(2):137-146. doi: 10.1007/s12275-022-1428-1. Epub 2021 Nov 26.
Four novel Gram-negative, mesophilic, aerobic, motile, and cocci-shaped strains were isolated from tick samples (strains 546 and 573) and respiratory tracts of marmots (strains 1318 and 1311). The 16S rRNA gene sequencing revealed that strains 546 and 573 were 97.8% identical to Roseomonas wenyumeiae Z23, whereas strains 1311 and 1318 were 98.3% identical to Roseomonas ludipueritiae DSM 14915. In addition, a 98.0% identity was observed between strains 546 and 1318. Phylogenetic and phylogenomic analyses revealed that strains 546 and 573 clustered with R. wenyumeiae Z23, whereas strains 1311 and 1318 grouped with R. ludipueritiae DSM 14915. The average nucleotide identity between our isolates and members of the genus Roseomonas was below 95%. The genomic G+C content of strains 546 and 1318 was 70.9% and 69.3%, respectively. Diphosphatidylglycerol (DPG) and phosphatidylethanolamine (PE) were the major polar lipids, with Q-10 as the predominant respiratory quinone. According to all genotypic, phenotypic, phylogenetic, and phylogenomic analyses, the four strains represent two novel species of the genus Roseomonas, for which the names Roseomonas haemaphysalidis sp. nov. and Roseomonas marmotae sp. nov. are proposed, with 546 (= GDMCC 1.1780 = JCM 34187) and 1318 (= GDMCC 1.1781 = JCM 34188) as type strains, respectively.
从蜱样本（菌株 546 和 573）和旱獭呼吸道中分离到四株新型革兰氏阴性、中温、需氧、运动和球菌状菌株。16S rRNA 基因测序结果表明，菌株 546 和 573 与玫瑰单胞菌 Z23 的相似度为 97.8%，而菌株 1311 和 1318 与玫瑰单胞菌 ludipueritiae DSM 14915 的相似度为 98.3%。此外，菌株 546 和 1318 之间存在 98.0%的同一性。系统发育和系统基因组分析表明，菌株 546 和 573 与玫瑰单胞菌 Z23 聚类，而菌株 1311 和 1318 与玫瑰单胞菌 ludipueritiae DSM 14915 聚类。我们的分离株与玫瑰单胞菌属成员之间的平均核苷酸同一性低于 95%。菌株 546 和 1318 的基因组 G+C 含量分别为 70.9%和 69.3%。二磷脂酰甘油（DPG）和磷脂酰乙醇胺（PE）是主要的极性脂，其中 Q-10 是主要的呼吸醌。根据所有基因型、表型、系统发育和系统基因组分析，这四株菌代表了玫瑰单胞菌属的两个新种，分别命名为血红玫瑰单胞菌和旱獭玫瑰单胞菌。546（=GDMCC 1.1780=JCM 34187）和 1318（=GDMCC 1.1781=JCM 34188）分别为模式菌株。
