Biotechnology Research Division, National Institute of Fisheries Science (NIFS), Gijang, Busan 46083, Republic of Korea.
Jeju Fisheries Research Institute, National Institute of Fisheries Science (NIFS), Jeju 63610, Republic of Korea.
Int J Syst Evol Microbiol. 2021 Nov;71(11). doi: 10.1099/ijsem.0.005085.
A Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacterial strain, MYP1-1, was isolated from the intestine of a stalked sea squirt () of the South Sea in the Republic of Korea. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain MYP1-1 clustered with the type strains of species and . Strain MYP1-1 exhibited 16S rRNA gene sequence similarity values of 97.0-97.6 % to the type strains of , and . The phylogenetic tree based on genomic sequences showed that strain MYP1-1 formed a distinct branch separating it from the type strains of two species and and other taxa. The DNA G+C content of strain MYP1-1 from its genomic sequence was 55.0 mol%. Strain MYP1-1 contained Q-10 as the predominant ubiquinone and C 7 as the major fatty acid. The major polar lipids of strain MYP1-1 were phosphatidylcholine, phosphatidylglycerol, one unidentified lipid and one unidentified aminolipid. The differences in fatty acid and polar lipid profiles and other differential phenotypic properties made it reasonable to distinguish strain MYP1-1 from the genera and . On the basis of the polyphasic taxonomic investigations, we conclude that strain MYP1-1 constitutes a new genus and species within the class , for which the name gen. nov., sp. nov. is proposed. The type strain is MYP1-1 (=KCTC 82143=NBRC 114355).
一株革兰氏阴性、严格需氧、非运动和杆状的细菌菌株 MYP1-1 是从韩国南海的一种有柄海鞘（）的肠道中分离出来的。基于 16S rRNA 基因序列的邻接法系统发育树显示，菌株 MYP1-1 与 种和 种的模式菌株聚在一起。菌株 MYP1-1 与 种、和 的模式菌株的 16S rRNA 基因序列相似性值为 97.0-97.6%。基于基因组序列的系统发育树显示，菌株 MYP1-1 形成了一个独特的分支，将其与两个 种和 和其他分类单元的模式菌株分开。从其基因组序列中获得的菌株 MYP1-1 的 DNA G+C 含量为 55.0mol%。菌株 MYP1-1 含有 Q-10 作为主要的泛醌和 C7 作为主要的脂肪酸。菌株 MYP1-1 的主要极性脂为磷脂酰胆碱、磷脂酰甘油、一种未鉴定的脂和一种未鉴定的胺脂。脂肪酸和极性脂图谱以及其他差异表型特性的差异使得将菌株 MYP1-1 与 和 属区分开来是合理的。基于多相分类学研究，我们得出结论，菌株 MYP1-1 构成了一个新的属和种，属于 纲，因此建议使用新属名 和新种名 。其模式菌株是 MYP1-1（=KCTC 82143=NBRC 114355）。
