用于检测循环黑素瘤细胞的流通式微流控系统。
A flow-through microfluidic system for the detection of circulating melanoma cells.
机构信息
Department of Biological Systems Engineering, University of Wisconsin-Madison, 460 Henry Mall, Madison, WI, 53706, USA.
Department of Dermatology, School of Medicine and Public Health, University of Wisconsin-Madison, 1300 University Avenue, Madison, WI, 53706, USA.
出版信息
Biosens Bioelectron. 2019 Oct 1;142:111522. doi: 10.1016/j.bios.2019.111522. Epub 2019 Jul 17.
We report the fabrication of polyaniline nanofiber (PANI)-modified screen-printed electrode (PANI/SPE) incorporated in a poly-dimethylsiloxane (PDMS) microfluidic channel for the detection of circulating tumor cells. We employed this device to detect melanoma skin cancer cells through specific immunogenic binding of cell surface biomarker melanocortin 1 receptor (MC1R) to anti-MC1R antibody. The antibody-functionalized PANI/SPE was used in batch-continuous flow-through fashion. An aqueous cell suspension of ferri/ferrocyanide at a flow rate of 1.5 mL/min was passed over the immunosensor, which allowed for continuous electrochemical measurements. The sensor performed exceptionally well affording an ultralow limit of quantification of 1 melanoma cell/mL, both in buffer and when mixed with peripheral blood mononuclear cells, and the response was log-linear over the range of 10-9000 melanoma cells/10 mL.
我们报告了聚邻苯二胺纳米纤维 (PANI) 修饰的丝网印刷电极 (PANI/SPE) 的制备，该电极集成在聚二甲基硅氧烷 (PDMS) 微流控通道中，用于检测循环肿瘤细胞。我们通过黑色素皮质素 1 受体 (MC1R) 与抗 MC1R 抗体的特异性免疫结合，利用该装置检测黑色素瘤皮肤癌细胞。抗体功能化的 PANI/SPE 以批量连续流动方式使用。铁氰化铁的水性细胞悬浮液以 1.5 mL/min 的流速通过免疫传感器，允许进行连续的电化学测量。该传感器表现出色，在缓冲液中和与外周血单核细胞混合时，定量下限低至 1 个黑色素瘤细胞/mL，并且在 10-9000 个黑色素瘤细胞/10 mL 的范围内呈对数线性响应。
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