Department of Pharmaceutical Analysis, JSS College of Pharmacy, Udhagamandalam, JSS Academy of Higher Education & Research, Tamil Nadu, India.
Department of Pharmaceutical Analysis, JSS College of Pharmacy, Udhagamandalam, JSS Academy of Higher Education & Research, Tamil Nadu, India.
Food Chem. 2019 Dec 1;300:125188. doi: 10.1016/j.foodchem.2019.125188. Epub 2019 Jul 16.
An effective, simple and sensitive analytical method has been developed employing liquid chromatography coupled with tandem mass spectrometry and validated for estimation of five organophosphate pesticides at trace levels in six fruits and twelve vegetables. Plackett-Burman design and central composite design was used to screen and optimize the significant factors in modified QuEChERS (quick, easy, cheap, effective, rugged and safe) extraction method. The method evaluation was done by matrix-matched calibration with linearity ranging from 5 to 500 µg/L with a correlation coefficient more than 0.990. The detection and quantification limit ranged from 0.1 to 1.0 µg/kg and 0.5 to 5 µg/kg, respectively. The mean recoveries were in the range of 76.89-110.30 % with the relative standard deviation less than 13.26% for all pesticides. Further, the method developed was applied to analyze real samples cultivated in the hill areas of Nilgiris, South India.
已经开发出一种有效、简单和灵敏的分析方法，采用液相色谱-串联质谱法，可用于在六种水果和十二种蔬菜中痕量水平下对五种有机磷农药进行定量分析。采用 Plackett-Burman 设计和中心组合设计筛选和优化改良 QuEChERS（快速、简单、廉价、有效、耐用和安全）提取方法中的显著因素。该方法通过基质匹配校准进行评估，线性范围为 5 至 500 µg/L，相关系数大于 0.990。检测限和定量限分别为 0.1 至 1.0 µg/kg 和 0.5 至 5 µg/kg。所有农药的平均回收率在 76.89-110.30%范围内，相对标准偏差均小于 13.26%。此外，所开发的方法已应用于分析在印度南部尼尔吉里斯丘陵地区种植的实际样本。
