从哈萨克斯坦家畜中分离出的基因型。
Genotypes of isolated from domestic livestock in Kazakhstan.
作者信息
Abdybekova A M, Zhang Z, Sultanov A A, Abdibayeva A A, Zhaksylykova A A, Junisbayeva S M, Aubakirov M Zh, Akhmetova G D, Torgerson P R
机构信息
Kazakh State Veterinary Research Institute, Almaty, Kazakhstan.
Veterinary Research Institute of Animal Science Academy of Xinjiang Uygur Autonomous Region, Urumqi 830000, China.
出版信息
J Helminthol. 2019 Jul 24;94:e69. doi: 10.1017/S0022149X19000634.
The diversity and importance of Echinococcus species in domesticated animals in Kazakhstan are poorly understood. In this study, 17 cysts of Echinococcus were collected from cattle and a further 17 cysts from sheep. DNA was extracted from the individual cysts and used for polymerase chain reaction amplification of mitochondrial subunit 1 of the cox1 and nadh1 gene. Amplicon sequencing results revealed the presence of Echinococcus granulosus sensu stricto G1 in 15 cattle and 15 sheep, and G3 genotype from two cattle. Echinococcus canadensis (G6/G7 strain) was found in two cysts originating from sheep.
哈萨克斯坦家畜中棘球绦虫种类的多样性和重要性鲜为人知。在本研究中，从牛身上采集了17个棘球绦虫囊肿，从羊身上又采集了17个囊肿。从单个囊肿中提取DNA，并用于对细胞色素氧化酶亚基1（cox1）和烟酰胺腺嘌呤二核苷酸脱氢酶亚基1（nadh1）基因的线粒体亚基1进行聚合酶链反应扩增。扩增子测序结果显示，15头牛和15只羊中存在狭义细粒棘球绦虫G1，两头牛中存在G3基因型。在源自羊的两个囊肿中发现了加拿大棘球绦虫（G6/G7株）。
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