Beijing Engineering Research Center for BioNanotechnology, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for NanoScience and Technology, No. 11 Zhongguancun Beiyitiao, Beijing, 100190, P. R. China and University of Chinese Academy of Sciences, No. 19 A Yuquan Road, Shijingshan District, Beijing 100049, P. R. China.
Beijing Engineering Research Center for BioNanotechnology, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for NanoScience and Technology, No. 11 Zhongguancun Beiyitiao, Beijing, 100190, P. R. China.
Lab Chip. 2019 Aug 21;19(16):2750-2757. doi: 10.1039/c9lc00517j. Epub 2019 Jul 24.
Point-of-care (POC) medical assays provide critical information to guide clinical therapy for a broad range of medical scenarios, such as resource-poor settings and specialty departments in hospitals. Even though many types of POC assays can be done in automated devices, these POC assays typically cannot well accommodate the multiplexed detection of biomarkers where a large dynamic range is needed. Here, we report a POC assay, which is both automated and suitable for detecting multiple biomarkers with dynamic detection ranges. We call it a dynamic multiplexed immunoassay (DMI). We control the concentrations of capture antibodies and the intensity of the readout signal to dynamically modulate the detection range of immunoassays (pg mL to μg mL), leading to the multiplexed detection of C-reactive protein (CRP), procalcitonin (PCT), and interleukin 6 (IL-6) simultaneously in undiluted human serum samples. The POC assay allows the rapid and accurate detection of infection in patients.
即时检测（POC）医疗检测为广泛的医疗场景提供了关键信息，有助于指导临床治疗，例如资源匮乏的环境和医院的专科部门。尽管有许多类型的 POC 检测可以在自动化设备中进行，但这些 POC 检测通常无法很好地适应需要大动态范围的生物标志物的多重检测。在这里，我们报告了一种 POC 检测方法，它既自动化，又适合检测具有动态检测范围的多种生物标志物。我们称之为动态多重免疫测定（DMI）。我们控制捕获抗体的浓度和读出信号的强度，以动态调节免疫测定的检测范围（pg/mL 至 μg/mL），从而在未经稀释的人血清样本中同时对 C 反应蛋白（CRP）、降钙素原（PCT）和白细胞介素 6（IL-6）进行多重检测。该 POC 检测方法可实现对患者感染的快速准确检测。
