School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA, 30332, USA.
Department of Infectious Diseases, University of Georgia, Athens, GA, 30602, USA.
Biomed Microdevices. 2024 Jan 30;26(1):15. doi: 10.1007/s10544-024-00694-2.
There is a clinical need for differential diagnosis of the latent versus active stages of tuberculosis (TB) disease by a simple-to-administer test. Alpha-crystallin (Acr) and early secretory antigenic target-6 (ESAT-6) are protein biomarkers associated with the latent and active stages of TB, respectively, and could be used for differential diagnosis. We therefore developed a microneedle patch (MNP) designed for application to the skin to quantify Acr and ESAT-6 in dermal interstitial fluid by enzyme-linked immunosorbent assay (ELISA). We fabricated mechanically strong microneedles made of polystyrene and coated them with capture antibodies against Acr and ESAT-6. We then optimized assay sensitivity to achieve a limit of detection of 750 pg/ml and 3,020 pg/ml for Acr and ESAT-6, respectively. This study demonstrates the feasibility of an MNP-based ELISA for differential diagnosis of latent TB disease.
临床需要一种简单易用的检测方法来区分潜伏性和活动性结核病（TB）。α-晶体蛋白（Acr）和早期分泌性抗原靶-6（ESAT-6）分别是与潜伏性和活动性结核病相关的蛋白生物标志物，可用于鉴别诊断。因此，我们开发了一种微针贴片（MNP），设计用于贴敷在皮肤上，通过酶联免疫吸附试验（ELISA）定量检测真皮间质液中的 Acr 和 ESAT-6。我们制造了机械强度高的聚苯乙烯微针，并在上面涂覆了针对 Acr 和 ESAT-6 的捕获抗体。然后，我们优化了检测的灵敏度，使 Acr 和 ESAT-6 的检测限分别达到 750pg/ml 和 3020pg/ml。本研究证明了基于 MNP 的 ELISA 用于潜伏性结核病鉴别诊断的可行性。
