Liu Shi-Qi, Sun Qing-Lei, Sun Yuan-Yuan, Yu Chao, Sun Li
1Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, PR China.
2Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, PR China.
Int J Syst Evol Microbiol. 2018 Aug;68(8):2538-2544. doi: 10.1099/ijsem.0.002870. Epub 2018 Jun 22.
In this study, we reported a novel yellow-pigmented, Gram-stain-negative bacterium with appendages, designated as strain L2, isolated from the South China Sea. Growth of strain L2 occurred at 22-40 °C (optimum, 37 °C), pH 6.0-10.0 (pH 7.0) and with 0-8 % (w/v) NaCl (2 %). Phylogenetic analysis based on its 16S rRNA gene sequence indicated that strain L2 belonged to the genus Muricauda. The close phylogenetic neighbours of strain L2 were Muricauda marina H19-56, Muricauda ruestringensis B1, Muricauda antarctica Ar-22, Muricauda taeanensis 105 and Muricauda flavescens SW-62 (96.4 %, 95.9 %, 95.9 %, 95.8 % and 94.5 % identities, respectively). The genomic DNA G+C content of strain L2 was 51.3±4.6 mol%. Theg major isoprenoid quinone was MK-6 (100.0 %). The polar lipids contained phosphatidylethanolamine and two unidentified lipids. The major fatty acids (>10 % of total fatty acids) were iso-C17 : 0 3-OH (30.3 %), iso-C15 : 1 G (20.6 %) and iso-C15 : 0 (17.6 %). Phylogenetic, physiological, biochemical and morphological analysis suggested that this strain represents a novel species of genus Muricauda, and the name Muricauda iocasae sp. nov. is proposed with the type species L2 (=CCTCC AB 2017193 =KCTC 62196).
在本研究中，我们报道了从中国南海分离出的一株带有附属物的新型黄色色素、革兰氏染色阴性细菌，命名为菌株L2。菌株L2在22-40°C（最适温度为37°C）、pH 6.0-10.0（pH 7.0）以及含0-8%（w/v）NaCl（2%）的条件下生长。基于其16S rRNA基因序列的系统发育分析表明，菌株L2属于Muricauda属。菌株L2的近缘系统发育邻居为滨海Muricauda菌H19-56、鲁氏Muricauda菌B1、南极Muricauda菌Ar-22、泰安Muricauda菌105和微黄Muricauda菌SW-62（同源性分别为96.4%、95.9%、95.9%、95.8%和94.5%）。菌株L2的基因组DNA G+C含量为51.3±4.6 mol%。主要类异戊二烯醌为MK-6（100.0%）。极性脂质包含磷脂酰乙醇胺和两种未鉴定的脂质。主要脂肪酸（占总脂肪酸的>10%）为异-C17:0 3-OH（30.3%）、异-C15:1 G（20.6%）和异-C15:0（17.6%）。系统发育、生理、生化和形态学分析表明，该菌株代表Muricauda属的一个新物种，因此提出新名称海洋Muricauda菌（Muricauda iocasae sp. nov.），模式菌株为L2（=CCTCC AB 2017193 =KCTC 62196）。
