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sp. nov.,分离自传统中国酸奶。

sp. nov., isolated from traditional Chinese yogurt.

机构信息

College of Life Sciences, Northeast Agricultural University, Harbin 150030, PR China.

Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, PR China.

出版信息

Int J Syst Evol Microbiol. 2020 Apr;70(4):2485-2492. doi: 10.1099/ijsem.0.004062. Epub 2020 Feb 25.

Abstract

Three Gram-stain-positive bacterial strains, designated X0750, X0278 and X0401, isolated from traditional yogurt in Tibet Autonomous Region, PR China, were characterized by a polyphasic approach, including sequence analyses of the 16S rRNA gene and three housekeeping genes (, and ), determination of average nucleotide identity (ANI) and average amino acid identity (AAI), DNA-DNA hybridization (DDH), fatty acid methyl ester (FAME) analysis and phenotypic characterization. Strain X0750 was phylogenetically related to the type strains of , , , , , , and , having 94.4-100 % 16S rRNA gene sequence similarities, 76.7-90.0 %  gene sequence similarities, 88.9-99.4 %  gene sequence similarities and 77.6-92.8 %  gene sequence similarities, respectively. ANI, DDH and AAI values between strain X0750 and type strains of phylogenetically related species were less than 90.4, 40.9 and 92.8 % respectively, confirming that strain X0750 represents a novel species within the genus . Based upon the data obtained in the present study, a novel species, sp. nov., is proposed and the type strain is X0750(=NCIMB 15192=CCM 8924=LMG 31184=CCTCC AB 2018403).

摘要

三株革兰氏阳性细菌菌株,命名为 X0750、X0278 和 X0401,从中国西藏自治区的传统酸奶中分离得到,采用多相分类法进行了特征描述,包括 16S rRNA 基因和三个管家基因(、和)序列分析、平均核苷酸同一性(ANI)和平均氨基酸同一性(AAI)测定、DNA-DNA 杂交(DDH)、脂肪酸甲酯(FAME)分析和表型特征。菌株 X0750与种的模式菌株、、、、、、和亲缘关系最密切,16S rRNA 基因序列相似度为 94.4-100%,基因序列相似度为 76.7-90.0%,基因序列相似度为 88.9-99.4%,基因序列相似度为 77.6-92.8%。与系统发育上相关的种的模式菌株相比,菌株 X0750 的 ANI、DDH 和 AAI 值分别小于 90.4%、40.9%和 92.8%,证实菌株 X0750 代表了属内的一个新种。基于本研究获得的数据,提出了一个新的种,即 sp. nov.,其模式菌株为 X0750(=NCIMB 15192=CCM 8924=LMG 31184=CCTCC AB 2018403)。

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