College of Life Sciences, Northeast Agricultural University, Harbin 150030, PR China.
Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, PR China.
Int J Syst Evol Microbiol. 2020 Apr;70(4):2485-2492. doi: 10.1099/ijsem.0.004062. Epub 2020 Feb 25.
Three Gram-stain-positive bacterial strains, designated X0750, X0278 and X0401, isolated from traditional yogurt in Tibet Autonomous Region, PR China, were characterized by a polyphasic approach, including sequence analyses of the 16S rRNA gene and three housekeeping genes (, and ), determination of average nucleotide identity (ANI) and average amino acid identity (AAI), DNA-DNA hybridization (DDH), fatty acid methyl ester (FAME) analysis and phenotypic characterization. Strain X0750 was phylogenetically related to the type strains of , , , , , , and , having 94.4-100 % 16S rRNA gene sequence similarities, 76.7-90.0 % gene sequence similarities, 88.9-99.4 % gene sequence similarities and 77.6-92.8 % gene sequence similarities, respectively. ANI, DDH and AAI values between strain X0750 and type strains of phylogenetically related species were less than 90.4, 40.9 and 92.8 % respectively, confirming that strain X0750 represents a novel species within the genus . Based upon the data obtained in the present study, a novel species, sp. nov., is proposed and the type strain is X0750(=NCIMB 15192=CCM 8924=LMG 31184=CCTCC AB 2018403).
三株革兰氏阳性细菌菌株,命名为 X0750、X0278 和 X0401,从中国西藏自治区的传统酸奶中分离得到,采用多相分类法进行了特征描述,包括 16S rRNA 基因和三个管家基因(、和)序列分析、平均核苷酸同一性(ANI)和平均氨基酸同一性(AAI)测定、DNA-DNA 杂交(DDH)、脂肪酸甲酯(FAME)分析和表型特征。菌株 X0750与种的模式菌株、、、、、、和亲缘关系最密切,16S rRNA 基因序列相似度为 94.4-100%,基因序列相似度为 76.7-90.0%,基因序列相似度为 88.9-99.4%,基因序列相似度为 77.6-92.8%。与系统发育上相关的种的模式菌株相比,菌株 X0750 的 ANI、DDH 和 AAI 值分别小于 90.4%、40.9%和 92.8%,证实菌株 X0750 代表了属内的一个新种。基于本研究获得的数据,提出了一个新的种,即 sp. nov.,其模式菌株为 X0750(=NCIMB 15192=CCM 8924=LMG 31184=CCTCC AB 2018403)。