Faculty of Dentistry, Federal University of Alfenas-MG, Unifal-MG, Alfenas, Brazil.
Faculty of Dentistry of Araçatuba, State University of São Paulo (UNESP), Sao Paulo, Brazil.
Clin Oral Investig. 2020 May;24(5):1637-1650. doi: 10.1007/s00784-019-03018-4. Epub 2019 Jul 23.
The effect of leucocyte- and platelet-rich fibrin (L-PRF), associated with DBBM (deproteinized bovine bone mineral; Bio-Oss®) was investigated and compared with autogenous bone graft as a standard material for filling bone defects.
A defect of 5 mm in diameter was performed in 40 calvaria of rats. The animals were divided into 5 groups and received blood clot (CO), autogenous bone (AUT), DBBM (BIO), L-PRF, or DBBM associated with L-PRF (BIO-LPRF). After 4 and 8 weeks, bone regeneration was assessed by histometric and immunohistochemical analyses.
The highest mean percentage of bone formation found at 4 and 8 weeks was observed for the BIO-L-PRF group (54.0% ± 2.8 and 63.6% ± 2.2). The lowest mean percentage at 4 and 8 weeks was observed for the CO group (16.7% ± 2.5 and 20.5% ± 1.0). There was statistical similarity among the AUT, BIO, and L-PRF groups. The expressions OC, RUNX 2, and VEGF showed a favorable aspect in the formation of new bone for BIO-L-PRF. VEGF was the marker with the highest expression because it was related to the initial healing process, promoting the migration and proliferation of endothelial cells in the region of the defect. Even after weeks, VEGF maintained a moderate expression.
The association of L-PRF with DBBM improved bone repair when these biomaterials were inserted into the defects of the calvaria of rats.
This reinforces the good performance of bovine bone and L-PRF as filler materials, especially when associated.
研究并比较富白细胞和血小板纤维蛋白（L-PRF）与脱蛋白牛骨矿物质（DBBM；Bio-Oss®）联合使用对填充骨缺损的效果，将其与自体骨移植物这一标准材料进行对比。
在 40 只大鼠的颅骨上制作直径为 5mm 的缺损。将动物分为 5 组，分别接受血凝块（CO）、自体骨（AUT）、DBBM（BIO）、L-PRF 或 DBBM 联合 L-PRF（BIO-LPRF）治疗。在 4 周和 8 周后，通过组织学和免疫组织化学分析评估骨再生情况。
在 4 周和 8 周时，观察到 BIO-L-PRF 组的骨形成平均百分比最高（分别为 54.0%±2.8 和 63.6%±2.2）。在 4 周和 8 周时，CO 组的平均百分比最低（分别为 16.7%±2.5 和 20.5%±1.0）。AUT、BIO 和 L-PRF 组之间的差异无统计学意义。BIO-L-PRF 组中 OC、RUNX2 和 VEGF 的表达在新骨形成方面表现良好。VEGF 是表达最高的标志物，因为它与初始愈合过程有关，可促进缺损区域内皮细胞的迁移和增殖。即使在数周后，VEGF 仍保持适度表达。
将 L-PRF 与 DBBM 联合使用可改善颅骨缺损中这些生物材料的骨修复效果。
这进一步证实了牛骨和 L-PRF 作为填充材料的良好性能，尤其是在联合使用时。
