Aryan Arefeh, Bayat Mohammad, Bonakdar Shahin, Taheri Soudabeh, Haghparast Newsha, Bagheri Mohammad, Piryaei Abbas, Abdollahifar Mohammad-Amin
Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
National Cell Bank, Pasteur Institute of Iran, Tehran, Iran.
Cells Tissues Organs. 2018;206(6):317-329. doi: 10.1159/000501651. Epub 2019 Jul 24.
Burn wound treatment is difficult and one of the most challenging problems in the clinic. Researchers have examined the applications of mesenchymal stem cells as a cell-based therapy for skin regeneration. But the role of human bone marrow mesenchymal stem cell conditioned medium (hBM-MSC-CM) in the treatment of burn injury remains unclear. This research aims at detecting whether hBM-MSC-CM can increase the wound healing of deep second-degree burns in male rats. In this study, 32 adult male rats per each time point were randomly divided into four groups: (1) control group, (2) sham group (DMEM), (3) common treatment group (CT), and (4) conditioned media group (CM). A 3 × 3 cm circular burn was created on the back of the rats. On postsurgical days 7, 15, and 28, the wound closure area of each wound was measured and then the skin samples were removed and analyzed using stereological methods. Wound closure area was significantly increased in the CM and CT groups on the 15th and the 28th day after burn injury compared to the control and DMEM groups. The stereological parameters and immunohistochemistry analysis of the wounds revealed significantly improved healing in the CM group compared to the control and other groups. It is concluded that these findings indicate that hBM-MSC-CM promotes skin wound healing by increasing cell proliferation, regulating collagen synthesis and collagen composition, and inducing angiogenesis at the injury site.
烧伤创面治疗困难，是临床上最具挑战性的问题之一。研究人员已研究了间充质干细胞作为基于细胞的皮肤再生治疗方法的应用。但人骨髓间充质干细胞条件培养基（hBM-MSC-CM）在烧伤治疗中的作用仍不清楚。本研究旨在检测hBM-MSC-CM是否能促进雄性大鼠深二度烧伤创面的愈合。在本研究中，每个时间点的32只成年雄性大鼠被随机分为四组：（1）对照组，（2）假手术组（DMEM），（3）常规治疗组（CT），和（4）条件培养基组（CM）。在大鼠背部制作一个3×3 cm的圆形烧伤创面。在术后第7天、15天和28天，测量每个创面的伤口闭合面积，然后取下皮肤样本并使用体视学方法进行分析。与对照组和DMEM组相比，烧伤后第15天和28天，CM组和CT组的伤口闭合面积显著增加。创面的体视学参数和免疫组织化学分析显示，与对照组和其他组相比，CM组的愈合情况显著改善。结论是，这些发现表明hBM-MSC-CM通过增加细胞增殖、调节胶原蛋白合成和胶原蛋白组成以及诱导损伤部位的血管生成来促进皮肤创面愈合。
