Garcia-Carmona F, Cabanes J, Garcia-Canovas F
Departamento de Bioquimica, Facultad de Biologia, Universidad de Murcia, Espinardo, Spain.
Biochem Int. 1987 Jun;14(6):1003-13.
Mushroom tyrosinase catalyzes the oxidation of sinephrine showing a marked lag period during appearance of adrenochrome and simultaneously adrenaline accumulation in the reaction medium can be detected. The adrenaline accumulation follows a sigmoidal curve until a constant level of adrenaline is reached when the system is in the steady-state. These experimental results agree with a model of enzymatic catalysis that includes the chemical evolution of adrenoquinone and permit us to explain these phenomenon as well as the influence that enzyme and sinephrine concentration present on the lag period and the level of adrenaline accumulated in the steady-state.
蘑菇酪氨酸酶催化辛弗林的氧化反应，在肾上腺色素出现期间呈现出明显的延迟期，同时可检测到反应介质中肾上腺素的积累。肾上腺素的积累呈S形曲线，直到系统达到稳态时肾上腺素达到恒定水平。这些实验结果与包含肾上腺醌化学演化的酶催化模型一致，使我们能够解释这些现象以及酶和辛弗林浓度对延迟期和稳态时积累的肾上腺素水平的影响。
