Zhang Haiyan, Guo Haixiang
Department of Urology, The Second Affiliated Hospital of Kunming Medical University, Kunming Yunnan, China.
J Int Med Res. 2019 Aug;47(8):3898-3904. doi: 10.1177/0300060519862076. Epub 2019 Jul 25.
Prostate cancer (PCA) is the deadliest urological disease affecting men worldwide. Long noncoding RNA activated by DNA damage (NORAD) levels are increased in many cancer types, and induce cancer cell progression. However, little is known about the biological functions of NORAD in PCA.
In this work, the roles of NORAD in cell proliferation, migration, and apoptosis were examined by Cell Counting Kit-8, scratch wound, and annexin V-fluorescein isothiocyanate/propidium iodide staining assays, respectively, in PCA cell lines. Knockdown of NORAD was achieved by small interfering (si)RNA in PCA cell lines, and quantitative real-time PCR was used to detect the expression of NORAD.
Cell proliferation and migration rates were significantly lower in the siNORAD group than in the wild-type group, while the apoptosis level was significantly higher in the siNORAD group compared with the wild-type group.
These results suggest that NORAD promotes the proliferation and migration of PCA cells and inhibits their apoptosis.
前列腺癌（PCA）是全球影响男性的最致命泌尿系统疾病。DNA损伤激活的长链非编码RNA（NORAD）在多种癌症类型中水平升高，并诱导癌细胞进展。然而，关于NORAD在PCA中的生物学功能知之甚少。
在这项研究中，分别通过细胞计数试剂盒-8、划痕伤口和膜联蛋白V-异硫氰酸荧光素/碘化丙啶染色试验，检测NORAD在PCA细胞系中对细胞增殖、迁移和凋亡的作用。在PCA细胞系中通过小干扰（si）RNA实现NORAD敲低，并使用定量实时PCR检测NORAD的表达。
siNORAD组的细胞增殖和迁移率显著低于野生型组，而siNORAD组的凋亡水平与野生型组相比显著更高。
这些结果表明NORAD促进PCA细胞的增殖和迁移，并抑制其凋亡。
