Chen Feihu, Liu Hao, Xia Jie, Ding Xiaomin, Fan Jianbo, Zhu Xinhui, Cui Shengyu, Yi Hong, Gao Rufeng, Liu Wei
School of Medicine, Nantong University, Nantong, Jiangsu, China.
Department of Orthopaedics, the Second Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.
J Int Med Res. 2019 Sep;47(9):4422-4430. doi: 10.1177/0300060519862460. Epub 2019 Jul 25.
Mounting evidence demonstrates that inflammation plays an important role in the pathogenesis of osteoarthritis (OA). Milk fat globule–epidermal growth factor 8 (MFG-E8) is an important glycoprotein that is involved in anti-inflammatory responses. The present study was performed to assess the MFG-E8 levels in plasma and synovial fluid and explore the association between radiographic severity and MFG-E8 levels in patients with knee OA.
This study involved 138 healthy controls and 142 patients with knee OA. The MFG-E8 levels in plasma and synovial fluid were evaluated by enzyme-linked immunosorbent assay. The Kellgren and Lawrence classification was used for OA grading.
The plasma MFG-E8 level was significantly lower in patients with knee OA than in healthy controls. The synovial fluid MFG-E8 level was significantly lower than the plasma level in patients with knee OA. More importantly, the MFG-E8 levels in synovial fluid and plasma were significantly and inversely associated with radiographic severity among patients with knee OA.
These results demonstrate that the levels of MFG-E8 in synovial fluid and plasma are inversely correlated with the radiographic severity of knee OA.
越来越多的证据表明炎症在骨关节炎（OA）的发病机制中起重要作用。乳脂肪球表皮生长因子8（MFG-E8）是一种参与抗炎反应的重要糖蛋白。本研究旨在评估血浆和滑液中MFG-E8的水平，并探讨膝关节OA患者影像学严重程度与MFG-E8水平之间的关联。
本研究纳入了138名健康对照者和142名膝关节OA患者。采用酶联免疫吸附测定法评估血浆和滑液中MFG-E8的水平。使用凯尔格伦和劳伦斯分类法对OA进行分级。
膝关节OA患者的血浆MFG-E8水平显著低于健康对照者。膝关节OA患者的滑液MFG-E8水平显著低于血浆水平。更重要的是，膝关节OA患者滑液和血浆中的MFG-E8水平与影像学严重程度呈显著负相关。
这些结果表明，滑液和血浆中MFG-E8的水平与膝关节OA的影像学严重程度呈负相关。
