Mabey Thomas, Honsawek Sittisak, Tanavalee Aree, Wilairatana Vajara, Yuktanandana Pongsak, Saetan Natthaphon, Zhan Dong
Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok 10330, Thailand.
Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok 10330, Thailand; Department of Orthopaedics, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok 10330, Thailand.
Clin Biochem. 2014 May;47(7-8):547-51. doi: 10.1016/j.clinbiochem.2014.03.011. Epub 2014 Mar 27.
The purpose of this study was to analyze sclerostin in plasma and synovial fluid of knee osteoarthritis (OA) patients and to investigate the association between sclerostin levels and radiographic severity.
A total of 190 subjects (95 knee OA patients and 95 healthy controls) were recruited in the present study. Sclerostin levels in plasma and synovial fluid were assessed using an enzyme-linked immunosorbent assay. OA grading was performed using the Kellgren-Lawrence classification.
Plasma sclerostin levels were significantly lower in OA patients than in healthy controls (P=0.004). Additionally, sclerostin levels in plasma were significantly higher with respect to paired synovial fluid (P<0.001). Moreover, sclerostin levels in plasma and synovial fluid demonstrated a significant inverse correlation with the radiographic severity of knee OA (r=-0.464, P<0.001 and r=-0.592, P<0.001, respectively). Subsequent analysis revealed that there was a positive correlation between plasma and synovial sclerostin levels (r=0.657, P<0.001).
Sclerostin was significantly lower in OA plasma samples when compared with healthy controls. Plasma and synovial fluid sclerostin levels were inversely associated with the radiographic severity of knee OA. Therefore, sclerostin may be utilized as a biochemical marker for reflecting disease severity in primary knee OA.
本研究旨在分析膝关节骨关节炎（OA）患者血浆和滑液中的硬化素，并探究硬化素水平与影像学严重程度之间的关联。
本研究共招募了190名受试者（95名膝关节OA患者和95名健康对照）。采用酶联免疫吸附测定法评估血浆和滑液中的硬化素水平。使用凯尔格伦-劳伦斯分类法进行OA分级。
OA患者血浆中的硬化素水平显著低于健康对照（P = 0.004）。此外，血浆中的硬化素水平相对于配对的滑液显著更高（P < 0.001）。而且，血浆和滑液中的硬化素水平与膝关节OA的影像学严重程度呈显著负相关（分别为r = -0.464，P < 0.001和r = -0.592，P < 0.001）。后续分析显示，血浆和滑液中的硬化素水平呈正相关（r = 0.657，P < 0.001）。
与健康对照相比，OA血浆样本中的硬化素显著降低。血浆和滑液中的硬化素水平与膝关节OA的影像学严重程度呈负相关。因此，硬化素可作为反映原发性膝关节OA疾病严重程度的生化标志物。
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