Miller R L, Simms B W, Gould A R
Department of Surgical and Hospital Dentistry, School of Dentistry, University of Louisville, KY 40292.
J Oral Pathol. 1988 Feb;17(2):73-8. doi: 10.1111/j.1600-0714.1988.tb01510.x.
The discriminative value of toluidine blue vital staining for detecting oral premalignant lesions and carcinomas was tested in the hamster DMBA buccal pouch model. The stain technique was further tested for specificity using the turpentine liquid petrolatum (TLP) hamster cheek pouch model for hyperplasia. Although carcinomas frequently exhibited toluidine blue staining, a 27.8% false negative result was noted. Premalignant lesions (dysplasia and carcinoma in situ) seldom retained toluidine blue stain and yielded a histologically confirmed (95.2%) false negative result. DMBA induced papillomas yielded a 5% false positive result. The TLP model failed to produce either clinically or histologically evident hyperplastic/hyperkeratotic lesions.
在仓鼠二甲基苯并蒽（DMBA）颊囊模型中测试了甲苯胺蓝活体染色对检测口腔癌前病变和癌的鉴别价值。使用松节油液体凡士林（TLP）仓鼠颊囊增生模型进一步测试了染色技术的特异性。尽管癌经常表现出甲苯胺蓝染色，但发现有27.8%的假阴性结果。癌前病变（发育异常和原位癌）很少保留甲苯胺蓝染色，产生了经组织学证实的（95.2%）假阴性结果。DMBA诱导的乳头状瘤产生了5%的假阳性结果。TLP模型未能产生临床上或组织学上明显的增生/角化过度病变。
