Dhingra J K, Zhang X, McMillan K, Kabani S, Manoharan R, Itzkan I, Feld M S, Shapshay S M
Department of Otolaryngology Head and Neck Surgery, New England Medical Center, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
Laryngoscope. 1998 Apr;108(4 Pt 1):471-5. doi: 10.1097/00005537-199804000-00002.
Laser-induced fluorescence (LIF) of tissues depends on their biochemical and histomorphologic characteristics. LIF spectroscopic properties of 9,10-dimethyl-1,2-benzanthracene (DMBA)-induced precancerous and early cancerous lesions in a hamster buccal pouch mucosa model were studied. Fluorescence spectra from neoplastic lesions showed a characteristic fluorescence peak in the red region of the visible spectrum centered between 630 and 640 nm when excited with 410-nm light. Using this as a diagnostic criterion, 45 of 49 lesions studied were correctly diagnosed, including early dysplastic lesions. Follow-up study of four dysplastic lesions over 2 weeks revealed an increase in red fluorescence intensity. The findings of these experiments suggest that LIF spectroscopy may be a valuable noninvasive technique not only for early diagnosis of head and neck cancer, but also to probe a possible biochemical surrogate biomarker in the follow-up of suspected lesions.
组织的激光诱导荧光（LIF）取决于其生化和组织形态学特征。研究了9,10 - 二甲基 - 1,2 - 苯并蒽（DMBA）诱导的仓鼠颊囊黏膜模型中的癌前病变和早期癌性病变的LIF光谱特性。当用410 nm光激发时，肿瘤性病变的荧光光谱在可见光谱的红色区域显示出特征性荧光峰，中心位于630至640 nm之间。以此作为诊断标准，所研究的49个病变中有45个被正确诊断，包括早期发育异常病变。对4个发育异常病变进行的为期2周的随访研究显示红色荧光强度增加。这些实验结果表明，LIF光谱学可能是一种有价值的非侵入性技术，不仅可用于头颈部癌症的早期诊断，还可用于在疑似病变的随访中探测可能的生化替代生物标志物。
