Ownby C L, Aird S D, Kaiser I I
Department of Physiological Sciences, Oklahoma State University, Stillwater 74078-0353.
Toxicon. 1988;26(3):319-23. doi: 10.1016/0041-0101(88)90223-1.
Myotoxins from C. v. concolor venom were isolated by gel filtration. This crude myotoxin peak was subfractionated into either two or four subfractions by cation exchange FPLC, depending upon the source of the venom. When injected at 2 micrograms/g, crude concolor myotoxin caused vacuolation of mouse muscle cells typical of myotoxin a from C. v. viridis and crotamine from C. d. terrificus. All four subfractions showed qualitatively identical myotoxin activity. In double immunodiffusion studies, myotoxin a antiserum produced lines of identity when reacted with myotoxin a, crude concolor myotoxin and the four concolor subfractions. A second batch of material showed two major components when subfractionated by cation exchange FPLC. The more basic of these two components displayed approximately twice the i.v. lethality of the more acidic component. The LD50 for the basic component lies between 0.625 and 0.75 microgram/g while that of the acidic component falls between 1.00 and 1.25 micrograms/g.
通过凝胶过滤从三色矛头蝮毒液中分离出肌毒素。根据毒液来源，通过阳离子交换快速蛋白质液相色谱法将该粗肌毒素峰再细分为两个或四个亚组分。当以2微克/克的剂量注射时，粗制三色矛头蝮肌毒素会导致小鼠肌肉细胞出现空泡化，这是绿曼巴蛇的肌毒素a和鼓腹咝蝰的响尾蛇胺所特有的。所有四个亚组分在质量上均表现出相同的肌毒素活性。在双向免疫扩散研究中，肌毒素a抗血清与肌毒素a、粗制三色矛头蝮肌毒素和四个三色矛头蝮亚组分反应时产生了沉淀线。第二批材料在通过阳离子交换快速蛋白质液相色谱法细分时显示出两个主要成分。这两种成分中碱性更强的成分的静脉内致死率约为酸性更强成分的两倍。碱性成分的半数致死量在0.625至0.75微克/克之间，而酸性成分的半数致死量在1.00至1.25微克/克之间。
