Biological Sciences Program, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Center of Excellence in Environment and Plant Physiology, Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Genes (Basel). 2019 Jul 25;10(8):562. doi: 10.3390/genes10080562.
Unfavourable environmental conditions, including soil salinity, lead to decreased rice (Oryza sativa L.) productivity, especially at the reproductive stage. In this study, we examined 30 rice varieties, which revealed significant differences in the photosynthetic performance responses under salt stress conditions during the reproductive stage, which ultimately affected yield components after recovery. In rice with a correlation between net photosynthetic rate (P) and intercellular CO concentration (C) under salt stress, P was found to be negatively correlated with filled grain number after recovery. Applying stringent criteria, we identified 130,317 SNPs and 15,396 InDels between two "high-yield rice" varieties and two "low-yield rice" varieties with contrasting photosynthesis and grain yield characteristics. A total of 2,089 genes containing high- and moderate-impact SNPs or InDels were evaluated by gene ontology (GO) enrichment analysis, resulting in over-represented terms in the apoptotic process and kinase activity. Among these genes, 262 were highly expressed in reproductive tissues, and most were annotated as receptor-like protein kinases. These findings highlight the importance of variations in signaling components in the genome and these loci can serve as potential genes in rice breeding to produce a variety with salt avoidance that leads to increased yield in saline soil.
不利的环境条件，包括土壤盐度，会导致水稻（Oryza sativa L.）生产力下降，特别是在生殖阶段。在这项研究中，我们研究了 30 个水稻品种，这些品种在生殖阶段盐胁迫条件下的光合作用性能反应存在显著差异，这最终影响了恢复后的产量构成。在盐胁迫下净光合速率（P）与胞间 CO 浓度（C）之间存在相关性的水稻中，P 与恢复后填充粒数呈负相关。应用严格的标准，我们在两种“高产水稻”品种和两种具有相反光合作用和粒产量特征的“低产水稻”品种之间鉴定出 130317 个 SNP 和 15396 个 InDels。通过基因本体论（GO）富集分析对包含高影响和中影响 SNP 或 InDels 的 2089 个基因进行了评估，导致凋亡过程和激酶活性的过度表达术语。在这些基因中，有 262 个在生殖组织中高表达，大多数被注释为受体样蛋白激酶。这些发现强调了基因组中信号成分变异的重要性，这些基因座可以作为水稻育种中的潜在基因，以产生一种具有避盐特性的品种，从而在盐渍土壤中提高产量。
