National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China.
Joint International Research Laboratory of Food & Medicine Resource Function, Henan Province, Kaifeng 475004, China.
Biomed Res Int. 2019 Jul 2;2019:3176483. doi: 10.1155/2019/3176483. eCollection 2019.
Three lignans, phillyrin, forsythia ester A, and rosin--D-furan glucose, were isolated from which is a famous Traditional Chinese Medicine used for clearing heat and detoxifying, reducing swelling and dispersing knot, and dispersing wind heat. In this study, the effects of phillyrin, forsythia ester A, and rosin--D-furan glucose on insulin resistance of 3T3-L1 adipocytes were investigated by the method of glucose oxidase-peroxidase (GOD-POD) and the mechanism was assayed by the method of western blot. The results indicated that phillyrin, forsythia ester A, and rosin--D-furan glucose could improve the glucose uptake in 3T3-L1 adipocytes under insulin resistance (IR). Among them, phillyrin showed significant activity in increasing glucose consumption at the concentrations of 100 M and 200 M ( < 0.001). The mechanism of improving insulin resistance may be that phillyrin could raise the protein phosphorylation of IRS-1 and Akt and the expression levels of GLUT4 protein.
从栀子中分离得到三种木脂素，即京尼平苷、连翘酯苷 A 和松脂醇-D-呋喃葡萄糖苷，栀子是一种著名的中药，具有清热泻火、消肿散结、疏散风热的功效。本研究采用葡萄糖氧化酶过氧化物酶（GOD-POD）法研究京尼平苷、连翘酯苷 A 和松脂醇-D-呋喃葡萄糖苷对胰岛素抵抗 3T3-L1 脂肪细胞的作用，采用 Western blot 法探讨其作用机制。结果表明，京尼平苷、连翘酯苷 A 和松脂醇-D-呋喃葡萄糖苷均可改善胰岛素抵抗 3T3-L1 脂肪细胞的葡萄糖摄取。其中，京尼平苷在 100 μM 和 200 μM 浓度下显著增加葡萄糖消耗（<0.001）。改善胰岛素抵抗的机制可能是京尼平苷能提高 IRS-1 和 Akt 的蛋白磷酸化水平以及 GLUT4 蛋白的表达水平。
