Purification and characterization of DNA methyltransferases from Neisseria gonorrhoeae.
作者信息
Piekarowicz A, Yuan R, Stein D C
机构信息
Medical Biotechnology Center, University of Maryland, Baltimore, MD.
出版信息
Nucleic Acids Res. 1988 Jul 11;16(13):5957-72. doi: 10.1093/nar/16.13.5957.
Abstract
Three DNA methyltransferases, M.NgoAI, and M.NgoBI and M.NgoBII, free of any nuclease activities were isolated from Neisseria gonorrhoeae strains WR220 and MUG116 respectively. M.NgoAI recognizes the sequence 5' GGCC 3' and methylates the first 5' cytosine on both strands. M.NgoBI and M.NgoBII recognize 5' TCACC 3' and 5' GTAN5CTC 3' respectively. M.NgoBII methylates cytosine on only one strand to produce 5' GTAN5mCTC 3'.
摘要
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