gen. nov., sp. nov., isolated from acidic mine drainage.

A heterotrophic and acidophilic bacterial strain, G45-3, was isolated from acidic mine drainage sampled in Fujian Province, PR China. Cells of strain G45-3 were Gram-stain-negative, non-spore-forming, non-motile and rod-shaped. Catalase and oxidase activities were positive. Strain G45-3 grew aerobically at 20-45 °C (optimum, 37 °C) and at pH 2.5-5.0 (optimum, pH 4.0). Photosynthetic pigments were not produced. Analysis of 16S rRNA gene sequences showed that strain G45-3 was phylogenetically related to different members of the family , and the sequence identities to JCM 10600, G2-11 and ATCC 35887 were 95.9 , 95.3 and 95.3 %, respectively. Strain G45-3 contained ubiquinone-10 as its respiratory quinone. The major polar lipids were determined to be diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified aminophospholipid and an unidentified aminolipid. The predominant fatty acids were cyclo-Cω8, Cω7, C and C. The genome of G45-3 consists of one chromosome (3 907 406 bp) and three plasmids (68 344, 45 771 and 16 090 bp), with an average G+C content of 65.9 mol%. Based on the results of phenotypic and genomic analyses, it is concluded that strain G45-3 represents a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. is nominated as type species and its type strain is G45-3 (=CGMCC 1.16069=KCTC 62275).