University of Chinese Academy of Sciences, Beijing 100049, PR China.
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, PR China.
Int J Syst Evol Microbiol. 2019 Oct;69(10):3248-3255. doi: 10.1099/ijsem.0.003618. Epub 2019 Jul 29.
A heterotrophic and acidophilic bacterial strain, G45-3, was isolated from acidic mine drainage sampled in Fujian Province, PR China. Cells of strain G45-3 were Gram-stain-negative, non-spore-forming, non-motile and rod-shaped. Catalase and oxidase activities were positive. Strain G45-3 grew aerobically at 20-45 °C (optimum, 37 °C) and at pH 2.5-5.0 (optimum, pH 4.0). Photosynthetic pigments were not produced. Analysis of 16S rRNA gene sequences showed that strain G45-3 was phylogenetically related to different members of the family , and the sequence identities to JCM 10600, G2-11 and ATCC 35887 were 95.9 , 95.3 and 95.3 %, respectively. Strain G45-3 contained ubiquinone-10 as its respiratory quinone. The major polar lipids were determined to be diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified aminophospholipid and an unidentified aminolipid. The predominant fatty acids were cyclo-Cω8, Cω7, C and C. The genome of G45-3 consists of one chromosome (3 907 406 bp) and three plasmids (68 344, 45 771 and 16 090 bp), with an average G+C content of 65.9 mol%. Based on the results of phenotypic and genomic analyses, it is concluded that strain G45-3 represents a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. is nominated as type species and its type strain is G45-3 (=CGMCC 1.16069=KCTC 62275).
一株异养嗜酸细菌菌株 G45-3 从中国福建省的酸性矿山排水中分离得到。菌株 G45-3 的细胞革兰氏染色阴性，不形成孢子，不运动，呈杆状。细胞具有触酶和氧化酶活性。菌株 G45-3 能够在有氧条件下生长，最适生长温度为 37℃，最适生长 pH 值为 4.0。菌株 G45-3 不产生光合色素。16S rRNA 基因序列分析表明，菌株 G45-3 与不同属的不同成员亲缘关系密切，与 JCM 10600、G2-11 和 ATCC 35887 的序列相似性分别为 95.9%、95.3%和 95.3%。菌株 G45-3 含有泛醌-10 作为其呼吸醌。主要极性脂为二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、一种未鉴定的氨基磷脂和一种未鉴定的氨基脂。主要脂肪酸为环-Cω8、Cω7、C 和 C。G45-3 的基因组由一条染色体（3907406bp）和三条质粒（68344、45771 和 16090bp）组成，GC 含量为 65.9mol%。基于表型和基因组分析的结果，菌株 G45-3 代表一个新属的新种，建议采用新属名. gen. nov.，新种名. sp. nov.。 被命名为模式种，其模式菌株为 G45-3（=CGMCC 1.16069=KCTC 62275）。
